Baker P E
Immunex Corporation, Seattle, WA.
Vet Immunol Immunopathol. 1987 Dec;17(1-4):193-209. doi: 10.1016/0165-2427(87)90140-1.
We utilized a human IL2 probe to isolate bovine IL2 sequences from a lymph node cDNA library. Bovine IL2 was subsequently expressed in both bacteria and yeast. Using a rapid, two-step purification scheme, we have been able to isolate over 20 mg/l of homogenous bovine rIL2 secreted from the yeast. The availability of sizable quantities of bovine rIL2 should make it possible to ascertain potential therapeutic or prophylactic utility of this lymphokine in cattle.
我们利用人白细胞介素2（IL2）探针从淋巴结cDNA文库中分离牛IL2序列。随后牛IL2在细菌和酵母中均得到表达。采用快速两步纯化方案，我们已能够从酵母中分离出每升超过20毫克的纯牛重组IL2（rIL2）。大量牛rIL2的可得性应使确定这种淋巴因子在牛中的潜在治疗或预防用途成为可能。
