Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa, Japan.
Department of Material and Life Chemistry, Kanagawa University, Yokohama, Kanagawa, Japan.
Mar Biotechnol (NY). 2020 Dec;22(6):824-835. doi: 10.1007/s10126-020-10010-7. Epub 2020 Nov 26.
Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria are considered as prolific producers of bioactive compounds. Thus, knowledge of the diversity of MMCFF cyanobacteria as related to bioactive compound production has become very important. However, basic taxonomic studies of MMCFF cyanobacteria are lacking. Many cyanobacterial taxa are still misidentified or undescribed. In this study, a total of 32 cyanobacterial colonies from nine coastal regions of Okinawa Prefecture were investigated for a diversity assessment. A polyphasic approach including morphological and molecular studies based on 16S rRNA gene sequences was performed to characterize Okinawan MMCFF cyanobacteria. Both morphological and molecular phylogenetic results showed that MMCFF cyanobacteria from Okinawan coasts are very diverse. We found morphotypes of Lyngbya-like, Phormidium-like, and Leptolyngbya-like groups among Okinawan cyanobacterial samples. Genetically, samples were distributed in various clades in the phylogenetic tree, including within Moorena, Okeania, Caldora, Neolyngbya, Dapis, as well as several unknown clades. In addition, cytotoxic activities of three samples from Kiyan coast were tested against HeLa cells. All three crude extracts of these samples showed strong cytotoxic activity with IC < 1 μg/ml.
海洋宏观集落形成丝状（MMCFF）蓝藻被认为是生物活性化合物的丰富生产者。因此，了解与生物活性化合物生产相关的 MMCFF 蓝藻的多样性变得非常重要。然而，对 MMCFF 蓝藻的基础分类学研究还很缺乏。许多蓝藻分类群仍然被错误识别或未被描述。在这项研究中，对来自冲绳县九个沿海地区的 32 个蓝藻菌落进行了多样性评估。采用包括基于 16S rRNA 基因序列的形态和分子研究的多相方法来表征冲绳 MMCFF 蓝藻。形态学和分子系统发育学的结果都表明，来自冲绳海岸的 MMCFF 蓝藻非常多样化。我们在冲绳蓝藻样本中发现了类似于 Lyngbya、Phormidium 和 Leptolyngbya 的形态型。在遗传学上，样品在系统发育树的各个分支中分布，包括 Moorena、Okeania、Caldora、Neolyngbya、Dapis 以及几个未知的分支。此外，还测试了来自 Kiyan 海岸的三个样品对 HeLa 细胞的细胞毒性。这些样品的三个粗提物均表现出强烈的细胞毒性，IC < 1μg/ml。
