CIIMAR/CIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal.
PLoS One. 2013 Jul 29;8(7):e69562. doi: 10.1371/journal.pone.0069562. Print 2013.
Cyanobacteria are widely recognized as a valuable source of bioactive metabolites. The majority of such compounds have been isolated from so-called complex cyanobacteria, such as filamentous or colonial forms, which usually display a larger number of biosynthetic gene clusters in their genomes, when compared to free-living unicellular forms. Nevertheless, picocyanobacteria are also known to have potential to produce bioactive natural products. Here, we report the isolation of hierridin B from the marine picocyanobacterium Cyanobium sp. LEGE 06113. This compound had previously been isolated from the filamentous epiphytic cyanobacterium Phormidium ectocarpi SAG 60.90, and had been shown to possess antiplasmodial activity. A phylogenetic analysis of the 16S rRNA gene from both strains confirmed that these cyanobacteria derive from different evolutionary lineages. We further investigated the biological activity of hierridin B, and tested its cytotoxicity towards a panel of human cancer cell lines; it showed selective cytotoxicity towards HT-29 colon adenocarcinoma cells.
蓝细菌被广泛认为是生物活性代谢物的有价值来源。这些化合物中的大多数都是从所谓的复杂蓝细菌中分离出来的，例如丝状或群体形式的蓝细菌，与自由生活的单细胞形式相比，它们的基因组中通常具有更多的生物合成基因簇。然而，微蓝细菌也有可能产生具有生物活性的天然产物。在这里，我们报告了从海洋微蓝细菌 Cyanobium sp. LEGE 06113 中分离出的hierridin B。该化合物先前已从丝状附生蓝细菌 Phormidium ectocarpi SAG 60.90 中分离出来，并已被证明具有抗疟原虫活性。来自两种菌株的 16S rRNA 基因的系统发育分析证实，这些蓝细菌来自不同的进化谱系。我们进一步研究了 hierridin B 的生物学活性，并测试了它对一系列人类癌细胞系的细胞毒性；它对 HT-29 结肠腺癌细胞具有选择性细胞毒性。
