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环状 GOLPH3 及其结合蛋白 CBX7 对前列腺癌细胞增殖和凋亡的调节作用。

Regulation of circGOLPH3 and its binding protein CBX7 on the proliferation and apoptosis of prostate cancer cells.

机构信息

Chengdu Second People's Hospital, Chengdu 10017, P.R. China.

Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, P.R. China.

出版信息

Biosci Rep. 2020 Dec 23;40(12). doi: 10.1042/BSR20200936.

DOI:10.1042/BSR20200936
PMID:33245100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7736626/
Abstract

To clarify the mechanism of circGOLPH3 regulation on prostate cancer cells, we performed an overexpression and interference circGOLPH3 assay in prostate cancer cells PC-3 and then evaluated cellular viability, proliferation, cell cycle, and apoptosis of prostate cancer cells by MTT, CCK8, Edu stain, TUNEL stain, and flow cytometry. Binding proteins of CircGOLPH3 were identified by RNA pull-down, mass spectrometry, and RNA-binding protein immunoprecipitation (RIP) assays. The expressions of CircGOLPH3 and CBX7 were measured by qRT-PCR. The results showed that after overexpression of circGOLPH3, the proliferative capacity and the viability of PC-3cells were significantly improved, whereas apoptosis was inhibited. CircGOLPH3 could bind to the CBX7 protein that was highly expressed in the PC-3 cell. Additionally, a functional test on CBX7 showed that the CBX7 overexpression notably improved the proliferative capacity and the viability of PC-3 cells and decreased cellular apoptosis, which was consistent with the effects of circGOLPH3. The validated the present study that circGOLPH3 and its binding protein CBX7 can promote prostate cancer cell proliferation and inhibit apoptosis.

摘要

为了阐明 circGOLPH3 对前列腺癌细胞调控的机制，我们在前列腺癌细胞 PC-3 中进行了 circGOLPH3 的过表达和干扰实验，然后通过 MTT、CCK8、Edu 染色、TUNEL 染色和流式细胞术评估了前列腺癌细胞的细胞活力、增殖、细胞周期和凋亡。通过 RNA 下拉、质谱和 RNA 结合蛋白免疫沉淀（RIP）实验鉴定 CircGOLPH3 的结合蛋白。通过 qRT-PCR 测量 CircGOLPH3 和 CBX7 的表达。结果表明，circGOLPH3 过表达后，PC-3 细胞的增殖能力和活力显著提高，而凋亡受到抑制。CircGOLPH3 可以与在 PC-3 细胞中高表达的 CBX7 蛋白结合。此外，对 CBX7 的功能测试表明，CBX7 的过表达显著提高了 PC-3 细胞的增殖能力和活力，并降低了细胞凋亡，这与 circGOLPH3 的作用一致。验证了本研究表明 circGOLPH3 及其结合蛋白 CBX7 可以促进前列腺癌细胞的增殖并抑制凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/c191093cebb9/bsr-40-bsr20200936-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/ffaf62c1e93b/bsr-40-bsr20200936-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/5e4ce1c6281d/bsr-40-bsr20200936-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/2941141f8674/bsr-40-bsr20200936-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/dc3c86704df3/bsr-40-bsr20200936-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/0b77293e4799/bsr-40-bsr20200936-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/c191093cebb9/bsr-40-bsr20200936-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/ffaf62c1e93b/bsr-40-bsr20200936-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/5e4ce1c6281d/bsr-40-bsr20200936-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/2941141f8674/bsr-40-bsr20200936-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/dc3c86704df3/bsr-40-bsr20200936-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/0b77293e4799/bsr-40-bsr20200936-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ae0/7736626/c191093cebb9/bsr-40-bsr20200936-g6.jpg

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Transl Androl Urol. 2019 Dec;8(6):686-695. doi: 10.21037/tau.2019.11.10.
2
Screening and identification of epithelial-to-mesenchymal transition-related circRNA and miRNA in prostate cancer.前列腺癌中上皮-间充质转化相关 circRNA 和 miRNA 的筛选与鉴定。
Pathol Res Pract. 2020 Feb;216(2):152784. doi: 10.1016/j.prp.2019.152784. Epub 2019 Dec 19.
3
Circular RNA SMARCA5 correlates with favorable clinical tumor features and prognosis, and increases chemotherapy sensitivity in intrahepatic cholangiocarcinoma.
Oncol Lett. 2023 Nov 13;27(1):14. doi: 10.3892/ol.2023.14147. eCollection 2024 Jan.
4
Comprehensive analysis reveals the value of the expression of chromobox family members for bladder urothelial carcinoma prognosis.综合分析揭示了染色体盒家族成员的表达对膀胱尿路上皮癌预后的价值。
Oncol Lett. 2023 Mar 14;25(4):172. doi: 10.3892/ol.2023.13758. eCollection 2023 Apr.
5
Chromobox proteins in cancer: Multifaceted functions and strategies for modulation (Review).染色盒蛋白在癌症中的作用：多功能性及调控策略（综述）。
Int J Oncol. 2023 Mar;62(3). doi: 10.3892/ijo.2023.5484. Epub 2023 Feb 3.
6
Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.骨髓间充质干细胞衍生的外泌体携带的Circ_0006790通过与胰腺癌中的CBX7结合来调节S100A11 DNA甲基化。
Am J Cancer Res. 2022 May 15;12(5):1934-1959. eCollection 2022.
7
The Role and Clinical Potentials of Circular RNAs in Prostate Cancer.环状RNA在前列腺癌中的作用及临床潜力
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8
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J Clin Lab Anal. 2020 Apr;34(4):e23138. doi: 10.1002/jcla.23138. Epub 2019 Dec 27.
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Open Med (Wars). 2019 Nov 20;14:833-842. doi: 10.1515/med-2019-0097. eCollection 2019.
5
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J Cell Biochem. 2020 Mar;121(3):2118-2126. doi: 10.1002/jcb.28239. Epub 2019 Oct 17.
6
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Cell Death Dis. 2019 Oct 17;10(11):792. doi: 10.1038/s41419-019-2028-9.
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Oncogene. 2019 Nov;38(45):7060-7072. doi: 10.1038/s41388-019-0947-7. Epub 2019 Aug 13.
8
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9
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10
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