Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy.
Institute of Plant Sciences Paris-Saclay, UMR9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot Sorbonne Paris-Cité, Saclay Plant Sciences, 91405 Orsay, France.
Phytopathology. 2021 Jul;111(7):1129-1136. doi: 10.1094/PHYTO-09-20-0441-R. Epub 2021 Aug 17.
T6085 has been investigated for many years as a beneficial isolate for use in the biocontrol of Fusarium head blight (FHB) of wheat caused primarily by . Previous work focused on application of T6085 to wheat spikes at anthesis, whereas application to soil before or at sowing has received limited attention. In the present study, the competitive ability of T6085 on plant residues against was investigated. Results showed a significant reduction of wheat straw colonization by the pathogen and of the development of perithecia, not only when T6085 was applied alone but also in the presence of a isolate (7121), well known as a natural competitor on wheat plant residues. T6085 was able to endophytically colonize wheat roots, resulting in internal colonization of the radical cortex area, without reaching the vascular system, as confirmed by confocal microscopy. This intimate interaction with the plant resulted in a significant increase of the expression of the plant defense-related genes and . Taken together, competitive ability, endophytic behavior, and host resistance induction represent three important traits that can be of great use in the application of T6085 against FHB not only on spikes at anthesis but potentially also in soil before or at sowing.
T6085 多年来一直被研究作为一种有益的分离物，用于生物防治小麦赤霉病（FHB），主要由 引起。以前的工作主要集中在开花期将 T6085 应用于小麦穗上，而在播种前或播种时应用于土壤则受到较少关注。在本研究中，研究了 T6085 对植物残体上 的竞争能力。结果表明，不仅当单独施用 T6085 时，而且当存在一种已知的小麦植物残体上的天然竞争者（7121）时，病原菌对小麦秸秆的定殖和子囊壳的发育都显著减少。T6085 能够内生性地定殖小麦根，导致根皮层区域的内部定殖，而不会进入维管束系统，这一点通过共聚焦显微镜得到了证实。这种与植物的密切相互作用导致植物防御相关基因 和 的表达显著增加。综上所述，竞争能力、内生行为和宿主抗性诱导是三个重要的特征，它们在应用 T6085 防治 FHB 方面非常有用，不仅可以在开花期的穗上使用，而且还可以在播种前或播种时在土壤中使用。
