Federal state budgetary establishment «Federal center cardiovascular surgery» (Astrakhan).
Astrakhan State Medical University.
Klin Lab Diagn. 2020 Sep 17;65(10):607-610. doi: 10.18821/0869-2084-2020-65-10-607-610.
Comparative analysis of the impact of blood collection systems on the results of routine coagulation tests. The aim of this study was to conduct comparative analysis of the effects of blood collection systems on the results of routine coagulation tests. The study involved practically healthy women over 18 years of age female (n=30). Inclusion criteria: practically healthy individuals. Exclusion criteria: taking non-steroidal anti-inflammatory drugs, or oral contraceptives. Blood collection was carried out in the following systems: 1 system-Vacuette Premium,2 system- Improvacuter, 3 system- BD Vacutainer® Plus. Before and after centrifugation, visual assessment of tubes was performed.No signs for the presence of clots, hemolysis, or insufficient filling was observed. In each sample we measured prothrombin time (PV, sec), activated partial thromboplastin time (APTT, sec), and fibrinogen (F, g/l) by clotting method on the automatic coagulometer «Sta Compact» («Diagnostica Stago», France). The results of laboratory analyses were processed using parametric and non - parametric statistics using The Statistica 12.0 software. At the preanalytical laboratory stage, there were no differences when using systems for blood sampling: the clots and hemolysis were not detected in the samples, the level of filling of the sistems was sufficient. Comparative analysis revealed small, though statistically significant, differences for: PT when comparing Improvacuter wiht Vacuette Premium or BD Vacutainer®; APPT when comparing Improvacuter wiht Vacuette Premium. No differences were observed for Fg levels. Our results support the data available in the literature on the effects of blood-taking systems on hemostasis , which should be taken into account when purchasing blood collection systems.
比较不同采血系统对常规凝血检测结果的影响。本研究旨在对不同采血系统对常规凝血检测结果的影响进行比较分析。研究对象为 18 岁以上的健康成年女性（n=30）。纳入标准：健康个体。排除标准：服用非甾体抗炎药或口服避孕药。采血系统如下：1 系统-Vacuette Premium，2 系统-Improvacuter，3 系统-BD Vacutainer® Plus。在离心前后，对采血管进行目视评估。未观察到凝块、溶血或采血量不足的迹象。在每个样本中，我们使用凝固法在自动凝血仪«Sta Compact»（法国 Diagnostica Stago）上测量凝血酶原时间（PT，秒）、活化部分凝血活酶时间（APTT，秒）和纤维蛋白原（F，g/l）。使用 The Statistica 12.0 软件对实验室分析结果进行了参数和非参数统计处理。在实验室前阶段，使用不同的采血系统时没有差异：样本中未检测到凝块和溶血，系统的填充水平充足。比较分析显示，当比较 Improvacuter 与 Vacuette Premium 或 BD Vacutainer®时，PT 有较小但具有统计学意义的差异；当比较 Improvacuter 与 Vacuette Premium 时，APTT 有较小但具有统计学意义的差异。Fg 水平无差异。我们的结果支持文献中关于采血系统对止血功能影响的现有数据，在购买采血系统时应考虑这些数据。
