Every-other day fasting prevents memory impairment induced by high fat-diet: Role of oxidative stress.

Imbalance of diet consumption results in memory and learning deterioration. High-fat diet (HFD) causes neuronal damage and eventually cognitive impairment, which can be related to increasing oxidative stress in the brain. Using the every other day fasting (EODF) paradigm, as a method of dietary restriction is thought to provide protection of learning and memory in several experimental studies. In the current work, the preventive effect of EODF paradigm on memory impairment-induced by HFD was investigated. Adult male Wistar rats were fed with HFD using the EODF paradigm for six weeks. At the end of these six weeks, and while the previous treatment were continued, rats were examined for learning and memory (both the short-term and the long-term memory) using the radial arm water maze (RAWM). Oxidative stress in the brain, namely in the hippocampus was also assessed. Chronic administration of HFD induced impairment in both, short- and long- term memory that was prevented using EODF paradigm. Furthermore, EODF prevented HFD-induced decrease in the activities of the antioxidant enzymes, SOD and catalase along with reduction of glutathione (GSH) level and the ratio of reduced glutathione/oxidized glutathione (GSH/GSSG ratio). The EODF also inhibited rise in oxidized glutathione (GSSG) and thiobarbituric acid reactive substances (TBARS) seen with HFD. In conclusion, EODF ameliorated oxidative stress and memory impairment induced by chronic HFD. This probably, can be explained by the ability of EODF to normalize mechanisms involved in oxidative stress in the hippocampus.