Alzoubi Karem H, Khabour Omar F, Al-Awad Rafat M, Aburashed Zainah O
Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Physiol Behav. 2021 Feb 1;229:113263. doi: 10.1016/j.physbeh.2020.113263. Epub 2020 Nov 24.
Imbalance of diet consumption results in memory and learning deterioration. High-fat diet (HFD) causes neuronal damage and eventually cognitive impairment, which can be related to increasing oxidative stress in the brain. Using the every other day fasting (EODF) paradigm, as a method of dietary restriction is thought to provide protection of learning and memory in several experimental studies. In the current work, the preventive effect of EODF paradigm on memory impairment-induced by HFD was investigated. Adult male Wistar rats were fed with HFD using the EODF paradigm for six weeks. At the end of these six weeks, and while the previous treatment were continued, rats were examined for learning and memory (both the short-term and the long-term memory) using the radial arm water maze (RAWM). Oxidative stress in the brain, namely in the hippocampus was also assessed. Chronic administration of HFD induced impairment in both, short- and long- term memory that was prevented using EODF paradigm. Furthermore, EODF prevented HFD-induced decrease in the activities of the antioxidant enzymes, SOD and catalase along with reduction of glutathione (GSH) level and the ratio of reduced glutathione/oxidized glutathione (GSH/GSSG ratio). The EODF also inhibited rise in oxidized glutathione (GSSG) and thiobarbituric acid reactive substances (TBARS) seen with HFD. In conclusion, EODF ameliorated oxidative stress and memory impairment induced by chronic HFD. This probably, can be explained by the ability of EODF to normalize mechanisms involved in oxidative stress in the hippocampus.
饮食摄入不均衡会导致记忆和学习能力下降。高脂饮食（HFD）会导致神经元损伤并最终导致认知障碍，这可能与大脑中氧化应激增加有关。在一些实验研究中，隔日禁食（EODF）模式作为一种饮食限制方法，被认为能对学习和记忆起到保护作用。在当前的研究中，我们调查了EODF模式对高脂饮食诱导的记忆损伤的预防作用。成年雄性Wistar大鼠采用EODF模式喂食高脂饮食六周。在这六周结束时，且在继续之前的治疗时，使用放射状臂水迷宫（RAWM）对大鼠的学习和记忆（短期和长期记忆）进行检测。同时也评估了大脑（即海马体）中的氧化应激情况。长期给予高脂饮食会导致短期和长期记忆损伤，而EODF模式可预防这种损伤。此外，EODF可防止高脂饮食诱导的抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶活性降低，以及谷胱甘肽（GSH）水平和还原型谷胱甘肽/氧化型谷胱甘肽比值（GSH/GSSG比值）的降低。EODF还可抑制高脂饮食引起的氧化型谷胱甘肽（GSSG）和硫代巴比妥酸反应性物质（TBARS）的升高。总之，EODF可改善长期高脂饮食诱导的氧化应激和记忆损伤。这可能是由于EODF能够使海马体中参与氧化应激的机制正常化。
