INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Av. Alem 1253, 8000 Bahía Blanca, Buenos Aires, Argentina.
INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Av. Alem 1253, 8000 Bahía Blanca, Buenos Aires, Argentina.
Colloids Surf B Biointerfaces. 2021 Feb;198:111460. doi: 10.1016/j.colsurfb.2020.111460. Epub 2020 Nov 12.
It is well known that iron oxide magnetic nanoparticles (IONPs) have many potential utilities in biomedicine due to their unique physicochemical properties. With the aim to obtain multifunctional nanoparticles with potential uses for therapy and diagnosis (nanotheranostics), IONPs were synthesized by hydrothermal synthesis assisted by mannose. Two synthetic pathways were evaluated in order to obtain IONPs with suitable properties for biomedical applications. The formulation Mag@Man/H1 presented the best characteristics in terms of size and stability. Mag@Man/H1 was evaluated as: a) drug carrier, b) antioxidant activity, c) magnetic hyperthermia, d) contrast agent for MRI. To evaluate the point a), morin, a natural flavonoid with several pharmaceutical activities, was loaded on the nanoparticles. A high percentage of drug loading was achieved. In point b) it was determined that the carrier itself possess a high activity which increased in morin loaded nanoparticles. Point c) magnetocalorimetric evaluation were carried out at several field conditions. A specific absorption rate value of 121.4 W/gFe was achieved at 52.4 kA/m and 260 kHz and 8.8 W/gFe at 4 kA/m and 100 kHz. Regarding contrast capacity (point d), the r1 value found was close to some contrast agent based on manganese. Although the measured r2 value was quite smaller than other iron oxides, the achieved effect was strong enough to produce negative contrast. From these studies, it was concluded that Mag@Man/H1 could act as a multifunctional nanoplatform for oncological diseases treatments.
众所周知，由于其独特的物理化学性质，氧化铁磁性纳米粒子（IONPs）在生物医药中有许多潜在的用途。为了获得具有治疗和诊断用途（纳米治疗学）的多功能纳米粒子，我们采用甘露糖辅助水热合成法合成了 IONPs。为了获得适用于生物医学应用的具有合适性质的 IONPs，我们评估了两种合成途径。就尺寸和稳定性而言，制剂 Mag@Man/H1 表现出最佳特性。我们对 Mag@Man/H1 进行了以下评估：a）药物载体，b）抗氧化活性，c）磁热疗，d）MRI 对比剂。为了评估 a）点，我们将具有多种药物活性的天然黄酮类化合物 morin 载入纳米粒子中。实现了高载药量。在 b）点，确定载体本身具有较高的活性，而负载 morin 的纳米粒子的活性更高。在 c）点，我们在不同的磁场条件下进行了磁热测量评估。在 52.4 kA/m 和 260 kHz 以及 4 kA/m 和 100 kHz 条件下，获得了 121.4 W/gFe 的特定吸收率值。在 d）点（对比能力），发现的 r1 值接近某些基于锰的对比剂。尽管测量的 r2 值比其他氧化铁小得多，但所产生的效果足以产生负对比。从这些研究中可以得出结论，Mag@Man/H1 可以作为治疗肿瘤疾病的多功能纳米平台。
