聚羟基烷酸酯的合成与表征：工业木质素生物价值化的蛋白质基因组学和过程优化研究。
Polyhydroxyalkanoate synthesis and characterization: A proteogenomic and process optimization study for biovalorization of industrial lignin.
机构信息
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
CRDT, IIT Delhi, New Delhi, India.
出版信息
Bioresour Technol. 2021 Jan;320(Pt B):124439. doi: 10.1016/j.biortech.2020.124439. Epub 2020 Nov 23.
The strain Burkholderia sp. ISTR5 (R5) was studied for polyhydroxyalkanoate (PHA) production on Kraft lignin (KL) and lignosulfonate (LS) as substrates. During the initial screening, the maximum PHA mass fraction in biomass produced on KL and LS was 23% and 18%, respectively, at 96 h. PHA production on KL was further optimized using the Box-Behnken Design (BBD) model of Response Surface Methodology (RSM). After optimization, a 42.5% increase in PHA production and a 32.2% increase in the total cell biomass was observed. PHA was characterized by GC-MS, TEM, FTIR, NMR, and fluorescence microscopy. It was found to be a small chain length PHA with a copolymer of poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV). The degradation of PHBV was also studied using this strain; it was observed that R5 completely degraded PHBV in 120 h. Genomic and proteomic analysis of R5 revealed numerous enzymes for the metabolism of lignin degradation and PHA production.
研究了伯克霍尔德氏菌（ISTR5，R5）在 Kraft 木质素（KL）和木质素磺酸盐（LS）作为底物上生产聚羟基烷酸酯（PHA）的情况。在初步筛选过程中，在 96 小时时，在 KL 和 LS 上生产的 PHA 生物质中的最大质量分数分别为 23%和 18%。使用响应面法（RSM）的 Box-Behnken 设计（BBD）模型进一步优化了 KL 上的 PHA 生产。优化后，PHA 产量增加了 42.5%，总细胞生物质增加了 32.2%。通过 GC-MS、TEM、FTIR、NMR 和荧光显微镜对 PHA 进行了表征。结果表明，PHA 是一种短链长的 PHA，是聚（羟基丁酸酯-共-羟基戊酸酯）（PHBV）的共聚物。还研究了该菌株对 PHBV 的降解，结果表明 R5 在 120 小时内完全降解了 PHBV。对 R5 的基因组和蛋白质组分析表明，其具有许多用于木质素降解和 PHA 生产代谢的酶。
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