Bioenergy Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, affiliated to Anna University, Chennai, TN, India.
Environ Sci Pollut Res Int. 2021 Apr;28(13):15923-15933. doi: 10.1007/s11356-020-11754-4. Epub 2020 Nov 27.
Nitrogen and phosphorous are important nutritional regulators for the growth of cyanobacteria, thereby having a significant impact in bloom formation by toxic species. Usage of toxic cyanobacteria for increasing valuable metabolite production by nutrient manipulation is still unexplored. Hence, the current work is aimed to estimate and compare growth, pigment, and increased lipid production coupled with the identification of fatty acids between two toxic strains-Anabaena circinalis FSS 124 and Cylindrospermopsis raciborskii FSS 127 under various combinations of these two nutrients. Low level of nitrogen and phosphorous enhanced lipid content in both strains (˃ 20% and 30% respectively) and C. raciborskii, respectively. Lipid productivity in low phosphorous concentration (P0.5) was achieved significantly high in C. raciborskii. Similarly, a substantial amount of carotenoids was obtained at reduced nitrogen and phosphorous in C. raciborskii accompanied by lessened growth and Chl a concentration. Unlikely, enough biomass (˃ 2 g L) was produced at high nutrient levels in both species. Comparative statistical significance (p < 0.05) was found between two species regarding biomass production, chlorophyll concentration, lipid content, and productivity and between these factors in each species under both nutrient variations. FAME of Cylindrospermopsis is composed of saturated fatty acids (˃ 50%) and MUFA (˃ 25%) while Anabaena contains PUFA (˃ 21%) additionally. However, the study highlights C. raciborskii as potential lipid and carotenoid producer at nutrient stress and finds a novel way to utilize these cyanobacterial biomasses, which cause immense environmental hazards and life threats.
氮和磷是蓝藻生长的重要营养调节剂，因此对有毒物种的形成有很大影响。通过营养操纵利用有毒蓝藻来增加有价值代谢产物的生产仍然是未知的。因此，目前的工作旨在估计和比较两种有毒菌株——Anabaena circinalis FSS 124 和 Cylindrospermopsis raciborskii FSS 127 在这两种营养物质的不同组合下的生长、色素和增加的脂质生产，同时鉴定脂肪酸。低氮和低磷水平分别提高了两种菌株（分别为˃20%和 30%）的脂质含量和 C. raciborskii 的脂质含量。在低磷浓度（P0.5）下，C. raciborskii 的脂质生产力显著提高。同样，在 C. raciborskii 中，在减少氮和磷的情况下获得了大量的类胡萝卜素，同时生长和 Chl a 浓度降低。相反，在两种物种的高营养水平下都产生了足够的生物量（˃2 g L）。在两种物种之间，关于生物量生产、叶绿素浓度、脂质含量和生产力以及在两种营养物质变化下每种物种的这些因素之间，都发现了具有统计学意义的显著差异（p <0.05）。Cylindrospermopsis 的 FAME 由饱和脂肪酸（˃50%）和 MUFA（˃25%）组成，而 Anabaena 则含有多不饱和脂肪酸（˃21%）。然而，该研究强调了 C. raciborskii 在营养胁迫下作为潜在的脂质和类胡萝卜素生产菌，并找到了一种利用这些蓝藻生物质的新方法，这些生物质会对环境造成巨大危害并威胁生命。
