Gateway Medical Innovation Center, Shanghai, China.
College of Veterinary Medicine, China Agricultural University, Beijing, China.
Cryobiology. 2021 Feb;98:164-171. doi: 10.1016/j.cryobiol.2020.11.006. Epub 2020 Nov 25.
The therapeutic effects of cryotherapy on skin and subcutaneous tumors in dogs were retrospectively studied in 20 dogs with 37 tumor lesions, of which 30 were benign and seven were malignant. Our results showed that during follow-up, 94.5% of lesions were completely exfoliated, without relapse or metastasis (mean time = 245.7 days). To investigate the effects of cryotherapy, we compared histopathological observations and microstructural changes in healthy tissues and tumor tissues, before and after cryotherapy. After cryotherapy, both normal skin and tumor tissue exhibited edema and hyperemia, with inflammatory cell infiltration. The cell nuclei exhibited pyknosis, disintegration and necrosis, and tight junctions were decreased in size. Cell morphology was varied, along with fragmented cell nuclear envelopes, crenulated nuclei and indistinct and necrotic intracellular organelles. Vacuoles were apparent in the cytoplasm and intercellular desmosomes were absent. These observations suggested that cryosurgery inhibited skin and subcutaneous tumors via cold-induced injury to cells, and cellular microenvironment changes induced by apoptosis. The results suggested that cryosurgery prevented skin and subcutaneous tumors via cold-induced injury to cells, and cellular microenvironment changes induced by apoptosis. We believe these data will provide general cryotherapy guidance to scientists and veterinary surgeons.
回顾性研究了冷冻疗法对 20 只患有 37 个肿瘤病变的犬的皮肤和皮下肿瘤的治疗效果，其中 30 个为良性，7 个为恶性。我们的研究结果表明，在随访期间，94.5%的病变完全脱落，无复发或转移（平均时间=245.7 天）。为了研究冷冻疗法的效果，我们比较了冷冻疗法前后健康组织和肿瘤组织的组织病理学观察和微观结构变化。冷冻疗法后，正常皮肤和肿瘤组织均出现水肿和充血，伴有炎症细胞浸润。细胞核出现固缩、崩解和坏死，紧密连接变小。细胞形态多样，伴有核膜碎裂、皱缩核和不清晰及坏死的细胞内细胞器。细胞质中出现空泡，细胞间桥粒缺失。这些观察结果表明，冷冻手术通过冷诱导细胞损伤和细胞凋亡引起的细胞微环境变化来抑制皮肤和皮下肿瘤。研究结果表明，冷冻手术通过冷诱导细胞损伤和细胞凋亡引起的细胞微环境变化来预防皮肤和皮下肿瘤。我们相信这些数据将为科学家和兽医提供一般的冷冻治疗指导。
