Ferretti A, Flanagan V P, Reeves V B
United States Department of Agriculture, Beltsville Human Nutrition Research Center, Lipid Nutrition Laboratory, Maryland 20705.
Anal Biochem. 1987 Nov 15;167(1):174-80. doi: 10.1016/0003-2697(87)90149-7.
A stable isotope dilution assay for the major urinary metabolite of prostaglandins E1 and E2 (PGE-M) was developed. After extraction and purification, the compound was converted to the dimethyl ester bis(O-methyloxime)-trimethylsilyl ether derivative and quantified with the 13C-labeled dimethyl ester analog as internal standard. The interassay coefficient of variation was 0.8%, and the intraassay coefficient of variation ranged from 0.9 to 1.7%. The recovery of authentic, underivatized PGE-M added to urine was 99.5 +/- 3.2% (mean +/- SE, N = 9). The equation of the regression curve correlating the amounts added and recovered was Y = 1.05X - 5.84, with R = 0.998. The level of excreted metabolite in healthy male subjects was 13.86 +/- 1.46 micrograms/24 h (N = 24), in good agreement with data from other sources.
开发了一种用于前列腺素E1和E2主要尿代谢物（PGE-M）的稳定同位素稀释测定法。提取和纯化后，该化合物转化为二甲基酯双（O-甲基肟）-三甲基硅醚衍生物，并以13C标记的二甲基酯类似物作为内标进行定量。批间变异系数为0.8%，批内变异系数范围为0.9%至1.7%。添加到尿液中的未衍生化真实PGE-M的回收率为99.5±3.2%（平均值±标准误，N = 9）。添加量与回收量相关的回归曲线方程为Y = 1.05X - 5.84，R = 0.998。健康男性受试者中排泄代谢物的水平为13.86±1.46微克/24小时（N = 24），与其他来源的数据高度一致。
