Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA; Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, USA.
Department of Chemistry, Grand Valley State University, Allendale, MI, USA.
Diagn Microbiol Infect Dis. 2021 Feb;99(2):115242. doi: 10.1016/j.diagmicrobio.2020.115242. Epub 2020 Oct 16.
Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii (CR Ab). In most isolates, an acquired bla carbapenemase was found in addition to the intrinsic bla allele. The most commonly found allele was bla (n = 78/98). In some isolates, bla was found in addition to other carbapenemase alleles: bla (n = 12/78), bla (n = 2/78) and bla (n = 1/78). Surprisingly, 20% of isolates carried carbapenemases not routinely assayed for by rapid molecular diagnostic platforms, i.e., bla and bla; all had ISAba1 elements. In 8 CR Ab, bla or bla was the only carbapenemase. Both bla and its variant bla were each found in 6/98 isolates. The most prevalent ADC variants were bla (21%), bla (21%), and bla (26%). Complete combinations are reported.
成功治疗鲍曼不动杆菌感染需要早期和适当的抗菌治疗。这一过程的第一步是了解存在哪些β-内酰胺酶（bla）基因座以及它们的组合方式。因此，我们对 98 株耐碳青霉烯鲍曼不动杆菌（CR Ab）进行了 WGS 分析。在大多数分离株中，除了固有 bla 基因座外，还发现了获得性 bla 碳青霉烯酶。最常见的基因座是 bla（n=78/98）。在一些分离株中，除了其他碳青霉烯酶基因座外，还发现了 bla：bla（n=12/78）、bla（n=2/78）和 bla（n=1/78）。令人惊讶的是，20%的分离株携带常规快速分子诊断平台未检测到的碳青霉烯酶，即 bla 和 bla；所有这些都带有 ISAba1 元件。在 8 株 CR Ab 中，bla 或 bla 是唯一的碳青霉烯酶。bla 和其变体 bla 分别在 6/98 株分离株中发现。最常见的 ADC 变体是 bla（21%）、bla（21%）和 bla（26%）。报告了完整的组合。
