Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; Hangzhou Singclean Medical Products Co., Ltd., Hangzhou 310018, China.
Int J Biol Macromol. 2021 Jan 15;167:117-129. doi: 10.1016/j.ijbiomac.2020.11.154. Epub 2020 Nov 26.
A novel seedbed-like scaffold was firstly fabricated by the "frozen sectioning" processing method using Flammulina velutipes as a raw material. The Flammulina velutipes polysaccharides scaffold is composed of a natural structure imitating the "ground" (connected and aligned hollow tubes with porous walls). Meanwhile, its biologically active components include polysaccharides and proteins, mimicking the "plant nutrition" in the seedbed. To further optimize the ground and nutrition components, Flammulina velutipes polysaccharides-derived scaffolds (FPDSs) were fabricated via the treatment of original Flammulina velutipes polysaccharides scaffold (labeled FPS) by NaOH, cysteine (labeled as FPS/NaOH, FPS/Cys, respectively). FPDSs were characterized by SEM, FTIR, XRD, water absorption and retention, and mechanical evaluations. From the results, FPS/NaOH and FPS/Cys lost the characteristic big tubes of original strips and had higher water absorption capacities comparing to FPS. Simultaneously, FPS/NaOH had better ductility, FPS/Cys had showed increased stiffness. Biological activities of FPDSs were tested against different types of bacteria exhibiting excellent anti-bacterial activity, and FPS/NaOH and FPS/Cys had dramatically higher anti-bacterial activity than FPS. The cytocompatibility of FPDSs was evaluated utilizing mouse fibroblast cell line (L929), and all FPDSs showed good cytocompatibility. The FPDSs were further applied to a rat full-thickness skin wound model, and they all exhibited obviously accelerated re-epithelialization, among which FPS/NaOH showed the greatest efficiency. FPS/NaOH could shorten the wound-healing process as evidenced by dynamic alterations of the expression levels of specific stagewise markers in the healing areas. Similarly, FPS/NaOH can efficiently induce hair follicle regeneration in the healing skin tissues. In summary, FPDSs exhibit potential functions as seedbeds to promote the regeneration of the "seed" including hair follicles and injured skin, opening a new avenue for wound healing.
一种新型的类似于种子床的支架首先通过“冷冻切片”加工方法，以金针菇为原料制备而成。金针菇多糖支架由一种天然结构组成，模仿“地面”（具有多孔壁的连接和排列的空心管）。同时，其生物活性成分包括多糖和蛋白质，模仿种子床中的“植物营养”。为了进一步优化地面和营养成分，通过用 NaOH 和半胱氨酸处理原始金针菇多糖支架（分别标记为 FPS/NaOH 和 FPS/Cys），制备了金针菇多糖衍生的支架（FPDSs）。通过 SEM、FTIR、XRD、吸水率和保留率以及机械评估对 FPDSs 进行了表征。结果表明，与原始条带相比，FPS/NaOH 和 FPS/Cys 失去了特征大管，并且具有更高的吸水率。同时，FPS/NaOH 具有更好的延展性，FPS/Cys 显示出更高的刚性。FPDSs 的生物活性通过测试对不同类型的细菌表现出优异的抗菌活性来评估，并且 FPS/NaOH 和 FPS/Cys 比 FPS 具有更高的抗菌活性。通过小鼠成纤维细胞系（L929）评估 FPDSs 的细胞相容性，所有 FPDSs 均显示出良好的细胞相容性。将 FPDSs 进一步应用于大鼠全层皮肤创面模型，结果表明所有 FPDSs 均明显加速了再上皮化，其中 FPS/NaOH 的效果最佳。FPS/NaOH 可以缩短创面愈合过程，这可以通过愈合区域中特定阶段性标志物的表达水平的动态变化来证明。同样，FPS/NaOH 可以有效地诱导愈合皮肤组织中的毛囊再生。总之，FPDSs 作为种子床具有促进包括毛囊和受损皮肤在内的“种子”再生的潜力，为创面愈合开辟了新途径。
