Ma Sheng, Xu Jianxiong, Lai Ting, Xu Weina, Zhang Jing, Zhang Hongcai, Zhang Weiyi
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Key Laboratory for Veterinary and Biotechnology, Shanghai, China.
Front Nutr. 2022 Jun 21;9:934073. doi: 10.3389/fnut.2022.934073. eCollection 2022.
To investigate the effect of polysaccharides (FVPs) on mice intestinal inflammation, FVPs were extracted from (FV) using a solid anaerobic fermentation technique. The antioxidant and anti-inflammatory capacities of FVP and fermented FVP (FFVP) induced by lipopolysaccharide (LPS) were investigated and . The results showed that the yield of FFVP (9.44%) was higher than that of FVP (8.65%), but the molecular weight (MW) of FFVP (15,702 Da) was lower than that of FVP (15,961 Da). The antioxidant and anti-inflammatory capacities of FFVP were higher than that of FVP in preventing mice diarrhea, enhancing antioxidant capacities, and reducing the secretion and mRNA expression of interleukin-1β (IL-1β), IL-6, IL-18, and tumor necrosis factor-α (TNF-α). The anti-inflammatory mechanisms of FVP and FFVP were analyzed by inhibiting the activation of the NLRP3 signaling pathway using an LPS-induced mice model. This study indicated that FFVP could be used as a functional antioxidant, indicating a potential application in functional food and health products.
为研究茯苓多糖（FVPs）对小鼠肠道炎症的影响，采用固体厌氧发酵技术从茯苓（FV）中提取FVPs。研究了脂多糖（LPS）诱导的FVPs和发酵茯苓多糖（FFVP）的抗氧化和抗炎能力。结果表明，FFVP的得率（9.44%）高于FVPs（8.65%），但FFVP的分子量（MW）（15,702 Da）低于FVPs（15,961 Da）。在预防小鼠腹泻、增强抗氧化能力以及降低白细胞介素-1β（IL-1β）、IL-6、IL-18和肿瘤坏死因子-α（TNF-α）的分泌和mRNA表达方面，FFVP的抗氧化和抗炎能力高于FVPs。使用LPS诱导的小鼠模型，通过抑制NLRP3信号通路的激活来分析FVPs和FFVP的抗炎机制。本研究表明，FFVP可作为一种功能性抗氧化剂，在功能性食品和保健品中具有潜在的应用价值。
