Department of Chemistry and Biochemistry, Long Island University, Brooklyn, NY, USA.
Department of Pharmacology and Toxicology, Long Island University, Brooklyn, NY, USA.
Arch Oral Biol. 2021 Feb;122:104989. doi: 10.1016/j.archoralbio.2020.104989. Epub 2020 Nov 18.
To determine the mechanism of growth inhibition of Aggregatibacter actinomycetemcomitans by Maillard reaction products (MRP).
Growth and cell viabilities in the presence or absence of MRP were measured for both the rough and smooth variants of the bacteria. Effects of addition of ferrous and ferric ions on the inhibition of the bacteria by MRP were determined.
MRPs decreased the extent of complex formation of Chrome Azurol S with iron suggesting that MRPs can chelate iron effectively. The chelation causes growth inhibition of both the rough and smooth strains. At low concentrations of the inhibitor, lag time was extended by approximately 12 h while at high concentrations, cells were killed, decreasing cell viability by up to 8 orders of magnitude. Growth of both the rough and smooth strains could be restored to original level by addition of iron. For the rough strain, both ferrous and ferric ions could relieve the inhibition by MRP while for the smooth strain only ferrous ion was effective.
MRPs inhibit the growth of A. actinomycetemcomitans by chelating iron and the inhibition can be relieved by addition of iron.
确定美拉德反应产物（MRP）对伴放线放线杆菌生长抑制的作用机制。
在存在或不存在 MRP 的情况下，测量细菌粗糙型和光滑型的生长和细胞活力。确定添加亚铁和铁离子对 MRP 抑制细菌的影响。
MRP 降低了 Chrome Azurol S 与铁形成复合物的程度，表明 MRP 可以有效地螯合铁。这种螯合作用导致粗糙型和光滑型菌株的生长受到抑制。在抑制剂浓度较低的情况下，延迟时间延长了约 12 小时，而在高浓度下，细胞被杀死，细胞活力降低了多达 8 个数量级。通过添加铁可以将两种菌株的生长恢复到原始水平。对于粗糙型菌株，亚铁和铁离子都可以缓解 MRP 的抑制作用，而对于光滑型菌株，只有亚铁离子有效。
MRP 通过螯合铁来抑制伴放线放线杆菌的生长，添加铁可以缓解这种抑制作用。
