Laber B, Amrhein N
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Federal Republic of Germany.
Biochem J. 1987 Dec 1;248(2):351-8. doi: 10.1042/bj2480351.
The alanine analogue 1-aminoethylphosphinate [H3C-CH(NH2)-PO2H2] effectively inhibited anthocyanin synthesis in buckwheat hypocotyls and caused an increase in the concentrations of alanine and alanine-derived metabolites. Aminotransferase inhibitors partially alleviated the effects of the analogue. 1-Aminoethylphosphinate did not affect the growth of Klebsiella pneumoniae under anaerobic conditions, but under aerobic conditions it inhibited growth and caused the massive excretion of pyruvate. The analogue inhibited the pyruvate dehydrogenase complex in vitro in the presence of an aminotransferase activity. The transamination product of 1-aminoethylphosphinate, acetylphosphinate (H3C-CO-PO2H2), was found to inhibit the pyruvate dehydrogenase complex in a time-dependent reaction that followed first-order and saturation kinetics and required the presence of thiamin pyrophosphate.
丙氨酸类似物1-氨基乙基次膦酸[H3C-CH(NH2)-PO2H2]有效抑制了荞麦下胚轴中花青素的合成,并导致丙氨酸和丙氨酸衍生代谢物的浓度增加。转氨酶抑制剂部分缓解了该类似物的作用。1-氨基乙基次膦酸在厌氧条件下不影响肺炎克雷伯菌的生长,但在有氧条件下它会抑制生长并导致丙酮酸大量排泄。在转氨酶活性存在的情况下,该类似物在体外抑制丙酮酸脱氢酶复合物。发现1-氨基乙基次膦酸的转氨产物乙酰次膦酸(H3C-CO-PO2H2)在一个遵循一级和饱和动力学且需要硫胺素焦磷酸存在的时间依赖性反应中抑制丙酮酸脱氢酶复合物。