Tanabe Taishi, Fukuda Yurina, Kawashima Kazuhiko, Yamamoto Satomi, Kashimoto Takashige, Sato Hisaaki
Laboratory of Veterinary Microbiology, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
Aino Animal Hospital, Takao 85-5, Fukuroi, Shizuoka 437-0023, Japan.
J Vet Med Sci. 2021 Jan 21;83(1):158-161. doi: 10.1292/jvms.20-0182. Epub 2020 Nov 27.
Alpha-amanitin, one of the amatoxins in egg amanita, has a cyclic peptide structure, and was reported as having antiviral activity against several viruses. We investigated whether α-amanitin has antiviral activity against feline immunodeficiency virus (FIV). FL-4 cells persistently infected with FIV Petaluma were cultured with α-amanitin. Reverse transcriptase (RT) activity in the supernatant of FL-4 cells was significantly inhibited by α-amanitin. In addition, the production of FIV core protein in FL-4 cells was inhibited by α-amanitin when analyzed by western blotting. Furthermore, α-amanitin inhibited the transcription of FIV in real-time RT-PCR. These data suggested that α-amanitin showed anti-FIV activity by inhibiting the RNA transcription level.
α-鹅膏毒肽是毒鹅膏中的一种鹅膏毒素，具有环状肽结构，据报道对多种病毒具有抗病毒活性。我们研究了α-鹅膏毒肽是否对猫免疫缺陷病毒（FIV）具有抗病毒活性。用α-鹅膏毒肽培养持续感染FIV Petaluma的FL-4细胞。α-鹅膏毒肽显著抑制了FL-4细胞上清液中的逆转录酶（RT）活性。此外，通过蛋白质印迹分析发现，α-鹅膏毒肽抑制了FL-4细胞中FIV核心蛋白的产生。此外，α-鹅膏毒肽在实时逆转录聚合酶链反应中抑制了FIV的转录。这些数据表明，α-鹅膏毒肽通过抑制RNA转录水平显示出抗FIV活性。
