Polouliakh Natalia, Ludwig Vanessa, Meguro Akira, Kawagoe Tatsukata, Heeb Oliver, Mizuki Nobuhisa
Department of Ophthalmology and Visual Sciences, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Sony Computer Science Laboratories Inc., Tokyo, Japan.
Front Physiol. 2020 Nov 4;11:586843. doi: 10.3389/fphys.2020.586843. eCollection 2020.
Alpha-arbutin (4-hydroxyphenyl alpha-glucopyranoside) is a known inhibitor of tyrosinase in keratinocytes; however, its effect on other genes and pathways in other skin cells has not been thoroughly investigated. In this study, we investigate the mechanism of alpha-arbutin activity in human dermal fibroblast cultures for 48 h. Results showed that the oxidative stress pathway was activated as alpha-arbutin reduced reactive oxygen species. In addition, we found a high possibility of wound healing and the upregulation of the insulin-like growth factor 1 receptor (IFG1R) pathway. We also investigated the role of the NRF2 gene in mediating the alpha-arbutin response. comparative genomics analysis conducted using our original tool, SHOE, suggested transcription factors with a role in tumor suppression and toxicity response as candidates for regulating the alpha-arbutin-mediated pathway.
α - 熊果苷（4 - 羟基苯基α - 吡喃葡萄糖苷）是一种已知的角质形成细胞中酪氨酸酶的抑制剂；然而，其对其他皮肤细胞中其他基因和信号通路的影响尚未得到充分研究。在本研究中，我们研究了α - 熊果苷在人皮肤成纤维细胞培养物中作用48小时的活性机制。结果表明，随着α - 熊果苷减少活性氧，氧化应激信号通路被激活。此外，我们发现其具有促进伤口愈合的高度可能性以及胰岛素样生长因子1受体（IFG1R）信号通路的上调。我们还研究了NRF2基因在介导α - 熊果苷反应中的作用。使用我们原创工具SHOE进行的比较基因组学分析表明，具有肿瘤抑制和毒性反应作用的转录因子是调节α - 熊果苷介导信号通路的候选因子。
