Ahuja S K, Manickavel V, Amy R M, Russell J C
Department of Surgery, University of Alberta, Edmonton, Canada.
Diabetes Res. 1987 Nov;6(3):137-44.
Islets of Langerhans in sections from the tail of the pancreas of corpulent LA/N-cp rats and lean controls aged 1, 3, 6 and 9 mo were examined by immunocytochemistry and morphometrically using an automatic image analyzer. The corpulent rats had significantly greater islet volumes at all ages, although islet hypertrophy tended to plateau after 6 mo. By 12 mo age the architecture of the islets was disrupted with large islets fused and showing areas of fibrosis and deposits of hemosiderin. The volume density (v/v, %) of islets in the parenchyma was significantly increased at each age step in corpulent rats reaching over 20% at 9 mo, and was greater in corpulent than in lean rats at all ages. In the corpulent rats, B-cell volume density dramatically with age and at all ages was significantly greater in corpulent than in lean rats. A-cell volume density was significantly greater in the corpulent rats than in lean rats at 1 and 9 mo. The mean B:A cell ratio was greater in corpulent than in lean rats at 3, 6 and 9 mo. There were more D cells per islet in corpulent than in lean rats up to 9 mo. These changes in cell populations were paralleled by qualitative changes in islet morphology and cellular topography such as increasingly irregular islet shape in corpulent animals and by variations in plasma levels of insulin and glucagon. In this strain of rats, obesity is associated with major changes in pancreatic morphology and this correlates strongly with the susceptibility of the strain to atherosclerosis.
对肥胖的LA/N-cp大鼠和1、3、6和9月龄的瘦对照大鼠胰腺尾部切片中的胰岛进行免疫细胞化学检查，并使用自动图像分析仪进行形态计量分析。肥胖大鼠在所有年龄段的胰岛体积均显著更大，尽管胰岛肥大在6个月后趋于稳定。到12个月龄时，胰岛结构被破坏，大胰岛融合，出现纤维化区域和含铁血黄素沉积。肥胖大鼠实质中胰岛的体积密度（v/v，%）在每个年龄阶段均显著增加，9个月时超过20%，且在所有年龄段肥胖大鼠的胰岛体积密度均大于瘦大鼠。在肥胖大鼠中，B细胞体积密度随年龄显著变化，且在所有年龄段肥胖大鼠的B细胞体积密度均显著大于瘦大鼠。1个月和9个月时，肥胖大鼠的A细胞体积密度显著大于瘦大鼠。3、6和9个月时，肥胖大鼠的平均B:A细胞比值大于瘦大鼠。在9个月龄之前，肥胖大鼠每个胰岛中的D细胞比瘦大鼠更多。细胞群体的这些变化与胰岛形态和细胞拓扑结构的定性变化并行，如肥胖动物中胰岛形状越来越不规则，以及胰岛素和胰高血糖素血浆水平的变化。在这种大鼠品系中，肥胖与胰腺形态的主要变化有关，这与该品系对动脉粥样硬化的易感性密切相关。
