Grochowski Emil Tomasz, Godlewski Adrian, Pietrowska Karolina, Gosk Wioleta, Wojnar Malgorzata, Konopinska Joanna, Kretowski Adam, Ciborowski Michal, Dmuchowska Diana Anna
Department of Ophthalmology, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland.
Metabolomics and Proteomics Laboratory, Clinical Research Center, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland.
Int J Mol Sci. 2025 Sep 3;26(17):8557. doi: 10.3390/ijms26178557.
This study aims to evaluate aqueous humor (AH) and serum metabolite concentrations in myopic and control adults undergoing cataract surgery and to correlate them with axial length (AL) to gain a better insight into the pathogenesis at both the local and systemic levels. Ninety-three patients were assigned to the myopic (n = 52) and control (n = 41) groups. Venous blood and aqueous humor samples were collected and analyzed by means of targeted metabolomics based on liquid chromatography-tandem mass spectrometry. The univariate analysis has revealed distinct metabolomic profiles between the myopic and control groups in AH but not in serum. In the AH of myopic patients, symmetric dimethylarginine (SDMA) and taurine concentrations were significantly lower. SDMA and taurine levels in the AH correlated negatively with the AL. These findings account for the insight into the local metabolic alterations in the case of myopia, potentially leading to novel therapeutic targets.
本研究旨在评估接受白内障手术的近视和对照成年患者的房水（AH）和血清代谢物浓度，并将它们与眼轴长度（AL）相关联，以便更好地了解局部和全身水平的发病机制。93例患者被分为近视组（n = 52）和对照组（n = 41）。采集静脉血和房水样本，并通过基于液相色谱-串联质谱的靶向代谢组学进行分析。单变量分析显示，近视组和对照组在房水中有不同的代谢组学特征，但在血清中没有。在近视患者的房水中，对称二甲基精氨酸（SDMA）和牛磺酸浓度显著较低。房水中的SDMA和牛磺酸水平与眼轴长度呈负相关。这些发现有助于深入了解近视情况下的局部代谢改变，可能会带来新的治疗靶点。
