在具有玻璃体视网膜界面异常的眼中，水状液和玻璃体液中的过敏毒素浓度。
Anaphylatoxin concentration in aqueous and vitreous humor in the eyes with vitreoretinal interface abnormalities.
机构信息
Department of Ophthalmology, Fukushima Medical University School of Medicine, Fukushima City, Fukushima, Japan.
Department of Ophthalmology, Fukushima Medical University School of Medicine, Fukushima City, Fukushima, Japan.
出版信息
Exp Eye Res. 2020 Jun;195:108025. doi: 10.1016/j.exer.2020.108025. Epub 2020 Mar 26.
The complement system may be activated in the posterior segment of the eye with chorioretinal disease, which may be reflected to the concentration of anaphylatoxins in the aqueous humor. Little is known about the distribution of anaphylatoxins in the aqueous and vitreous humor. The aim of the present study was to investigate the distribution of anaphylatoxin concentration in the aqueous and vitreous humor of the eyes with idiopathic epiretinal membrane or idiopathic macular hole. This was an experimental, observational case series. This study included 43 eyes from 43 patients; 29 eyes with idiopathic epiretinal membrane, and 14 eyes with idiopathic macular hole. All 43 eyes underwent cataract surgery and vitrectomy. The aqueous and vitreous humor were collected at the surgery. The anaphylatoxin concentrations were measured by using a cytometric beads array, and the respective C3a, C4a, and C5a concentrations were 2.003 ± 0.679 (mean ± standard deviation) ng/ml, 1.389 ± 0.419 ng/ml, and 0.003 ± 0.004 ng/ml in the aqueous humor, and 1.236 ± 0.642 ng/ml, 1.250 ± 0.542 ng/ml, and 0.048 ± 0.069 ng/ml in the vitreous humor. The mean C3a concentration in the aqueous humor was significantly higher than in the vitreous humor in 43 eyes of iMH and iERM (P < 0.001). The mean C4a concentration showed no significant difference between the aqueous humor and vitreous humor (P = 0.282), and the mean C5a in the aqueous humor was significantly lower than in the vitreous humor overall (P < 0.001). The C3a concentration in the aqueous humor strongly correlated with that in the vitreous humor (R = 0.510, P < 0.001). The concentrations of C4a and C5a in the aqueous humor moderately correlated with those in the vitreous humor (C4a; R = 0.356, P = 0.019, C5a; R = 0.464, P = 0.022). In conclusion, the anaphylatoxin concentrations measured by cytometric beads array in the aqueous humor may be associated with those measured in the vitreous humor.
补体系统可能会在伴有脉络膜视网膜疾病的眼后段被激活，这可能反映在房水中过敏毒素的浓度上。关于过敏毒素在房水和玻璃体液中的分布知之甚少。本研究的目的是研究特发性视网膜前膜或特发性黄斑裂孔眼的房水和玻璃体液中过敏毒素浓度的分布。这是一项实验性观察性病例系列研究。本研究共纳入 43 例 43 只眼；29 只眼为特发性视网膜前膜，14 只眼为特发性黄斑裂孔。所有 43 只眼均行白内障手术联合玻璃体切除术。在手术中收集房水和玻璃体液。通过细胞因子微珠阵列法测量过敏毒素浓度，房水中的 C3a、C4a 和 C5a 浓度分别为 2.003±0.679（均值±标准差）ng/ml、1.389±0.419ng/ml 和 0.003±0.004ng/ml，玻璃体液中的 C3a、C4a 和 C5a 浓度分别为 1.236±0.642ng/ml、1.250±0.542ng/ml 和 0.048±0.069ng/ml。43 只 iMH 和 iERM 眼中，房水中 C3a 的平均浓度显著高于玻璃体液（P<0.001）。房水和玻璃体液中 C4a 的平均浓度无显著差异（P=0.282），房水中 C5a 的平均浓度总体上显著低于玻璃体液（P<0.001）。房水中 C3a 浓度与玻璃体液中 C3a 浓度呈强相关（R=0.510，P<0.001）。房水中 C4a 和 C5a 的浓度与玻璃体液中 C4a 和 C5a 的浓度呈中度相关（C4a；R=0.356，P=0.019，C5a；R=0.464，P=0.022）。总之，通过细胞因子微珠阵列法测量的房水中过敏毒素浓度可能与玻璃体液中的浓度相关。
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