HIV Netherlands Australia Thailand Research Collaboration (HIV-NAT), Thai Red Cross AIDS Research Centre, Bangkok, Thailand.
HIV Netherlands Australia Thailand Research Collaboration (HIV-NAT), Thai Red Cross AIDS Research Centre, Bangkok, Thailand.
Tuberculosis (Edinb). 2021 Jan;126:102017. doi: 10.1016/j.tube.2020.102017. Epub 2020 Nov 11.
A flow cytometric assay measuring Mycobacterium tuberculosis-specific CD4 T-cell responses using co-expression of CD25/CD134 (OX40 assay) was explored as a diagnostic tool for active tuberculosis (TB) in a Thai population with and without HIV infection. Peripheral blood mononuclear cells (PBMC) obtained from 133 participants at TB diagnosis were cryopreserved. Seventy-six participants had a clinical diagnosis of TB which were confirmed by a positive culture. CD4 T-cell responses were measured after stimulation with a pool of overlapping peptides covering RD-1 antigens: CFP-10 + ESAT-6. The performance of the assay was also compared to the Xpert MTB/RIF assay. The overall sensitivity of the OX40 assay was 94.7% (95%CI 87.1-98.5); its specificity was 71.9% (95%CI, 58.5-83). The sensitivity of the OX40 assay among HIV-infected participants was 100% (95%CI, 88.8-100) with a specificity of 92.9% (95%CI, 66.1-99.8). OX40 assay performed particularly well in those with active TB and HIV infection.
使用共表达 CD25/CD134（OX40 测定法）来测量结核分枝杆菌特异性 CD4 T 细胞应答的流式细胞术测定法被探索作为一种诊断工具，用于泰国人群中伴有和不伴有 HIV 感染的活动性肺结核（TB）。在 TB 诊断时从 133 名参与者中获得的外周血单核细胞（PBMC）被冷冻保存。76 名参与者具有 TB 的临床诊断，这些诊断通过阳性培养得到证实。用覆盖 RD-1 抗原的重叠肽池（CFP-10 + ESAT-6）刺激后测量 CD4 T 细胞应答。该测定法的性能也与 Xpert MTB/RIF 测定法进行了比较。OX40 测定法的总体敏感性为 94.7%（95%CI 87.1-98.5）；其特异性为 71.9%（95%CI，58.5-83）。在感染 HIV 的参与者中，OX40 测定法的敏感性为 100%（95%CI，88.8-100），特异性为 92.9%（95%CI，66.1-99.8）。OX40 测定法在伴有活动性 TB 和 HIV 感染的患者中表现尤其出色。
