Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi, 214122, China.
Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi, 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, Jiangnan University, Wuxi, 214122, China; Jiangsu College of Water Treatment Technology and Material Collaborative Innovation Center, Suzhou, 215009, China.
Environ Pollut. 2020 Dec;267:115644. doi: 10.1016/j.envpol.2020.115644. Epub 2020 Sep 18.
Foaming caused by filamentous bacteria in activated sludge (AS) is a common phenomenon in municipal wastewater treatment plants (WWTPs) in Taihu Lake Basin of South China. In this study, total bacterial and filamentous bacterial communities were comprehensively characterized in AS and foams from eight municipal WWTPs by high-throughput sequencing technology. Results showed that alpha diversities of total bacterial communities in foams were obviously lower than those in AS samples. The bacterial community structures were significantly different between WWTPs rather than sample types (AS vs. foam). For most WWTPs, the Actinobacteria phylum was highly enriched in foams and the most abundant genera in foams were common mycolata. Sixteen filamentous bacteria were identified against the improved bulking and foaming bacteria (BFB) database. Abundance and composition of BFB in different WWTPs and different sample types were significantly different. 'Nostocoida limicola' I Trichococcus and Microthrix were generally dominant in AS samples. The dominant BFB in foams were associated with Microthrix, Skermania, Gordonia, and Mycobacterium. A new Defluviicoccus spp. in cluster III was identified in severe and continuous foams. Moreover, dominant BFB in stable and continuous foams with light level in one typical WWTP were diverse, even, and dynamic. Bacterial co-occurrence network analysis implied that the bacterial community of AS was more sensitive to disturbance than that of foam.
在中国南方太湖流域的城市污水处理厂（WWTP）中，活性污泥（AS）中丝状菌引起的泡沫是一种常见现象。在本研究中，通过高通量测序技术全面描述了来自八个城市 WWTP 的 AS 和泡沫中的总细菌和丝状细菌群落。结果表明，泡沫中总细菌群落的α多样性明显低于 AS 样品。细菌群落结构在 WWTP 之间差异显著，而不是样品类型（AS 与泡沫）。对于大多数 WWTP，Actinobacteria 门在泡沫中高度富集，泡沫中最丰富的属是普通放线菌。针对改良的膨胀和泡沫细菌（BFB）数据库鉴定了 16 种丝状细菌。不同 WWTP 和不同样品类型的 BFB 丰度和组成差异显著。在 AS 样品中，' Nostocoida limicola' I Trichococcus 和 Microthrix 通常占优势。泡沫中占优势的 BFB 与 Microthrix、Skermania、Gordonia 和 Mycobacterium 有关。在严重且持续的泡沫中发现了簇 III 中的一种新的 Defluviicoccus spp.。此外，在一个典型 WWTP 中具有低水平稳定且持续的泡沫中，优势 BFB 是多样、均匀和动态的。细菌共现网络分析表明，AS 中的细菌群落比泡沫中的细菌群落对干扰更敏感。
