Dettori Maria Luisa, Petretto Elena, Pazzola Michele, Vidal Oriol, Amills Marcel, Vacca Giuseppe Massimo
Department of Veterinary Medicine, University of Sassari, via Vienna 2, 07100 Sassari, Italy.
Departament de Biologia, Universitat de Girona, 17003 Girona, Spain.
Animals (Basel). 2020 Nov 24;10(12):2194. doi: 10.3390/ani10122194.
A sample of 146 Sarda bucks from eight subregions of Sardinia, Italy (Nuorese, Barbagia, Baronia, Ogliastra, Sarrabus, Guspinese, Iglesiente, Sulcis) were characterized for Y-chromosome and mtDNA markers to assess the levels of population substructure. Five polymorphic loci (SRY, AMELY, ZFY, and DDX3Y) on the Y-chromosome were genotyped. The control region of mtDNA was sequenced as a source of complementary information. Analysis of Y-chromosome data revealed the segregation of 5 haplotypes: Y1A (66.43%), Y2 (28.57%), Y1C (3.57%), Y1B1 (0.71%), and Y1B2 (0.71%). High levels of Y-chromosome diversity were observed in populations from Southwest Sardinia. The F values based on Y-chromosome and mtDNA data were low, although a paternal genetic differentiation was observed when comparing the Nuorese and Barbagia populations (Central Sardinia) with the Sulcis, Iglesiente, and Sarrabus populations (Southern Sardinia). AMOVA analysis supported the lack of population substructure. These results suggest the occurrence of a historical and extensive gene flow between Sarda goat populations from different locations of Sardinia, despite the fact that this island is covered by several large mountain ranges. Introgression with foreign caprine breeds in order to improve milk production might have also contributed to avoiding the genetic differentiation amongst Sarda populations.
对来自意大利撒丁岛八个次区域（努奥罗、巴尔巴吉亚、巴罗尼亚、奥利亚斯特拉、萨拉布斯、古斯皮内塞、伊格莱西亚、苏尔西斯）的146只撒丁岛公羊样本进行了Y染色体和线粒体DNA标记特征分析，以评估种群亚结构水平。对Y染色体上的五个多态性位点（SRY、AMELY、ZFY和DDX3Y）进行了基因分型。对线粒体DNA的控制区进行了测序，作为补充信息来源。Y染色体数据分析揭示了5种单倍型的分离：Y1A（66.43%）、Y2（28.57%）、Y1C（3.57%）、Y1B1（0.71%）和Y1B2（0.71%）。在撒丁岛西南部的种群中观察到高水平的Y染色体多样性。基于Y染色体和线粒体DNA数据的F值较低，尽管在比较努奥罗和巴尔巴吉亚种群（撒丁岛中部）与苏尔西斯、伊格莱西亚和萨拉布斯种群（撒丁岛南部）时观察到父系遗传分化。AMOVA分析支持不存在种群亚结构。这些结果表明，尽管撒丁岛被几条大型山脉覆盖，但来自撒丁岛不同地点的撒丁岛山羊种群之间存在历史悠久且广泛的基因流动。为了提高产奶量而与外来山羊品种的基因渗入也可能有助于避免撒丁岛种群之间的遗传分化。
