UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, D02 YH21 Dublin 2, Ireland.
Cancer and Disease Epigenetics, Murdoch Children's Research Institute, Melbourne, VIC, Australia.
Nutrients. 2020 Nov 24;12(12):3602. doi: 10.3390/nu12123602.
A range of in utero and early-life factors can influence offspring epigenetics, particularly DNA methylation patterns. This study aimed to investigate the influence of a dietary intervention and factors in pregnancy on offspring epigenetic profile at five years of age. We also explored associations between body composition and methylation profile in a cross-sectional analysis. Sixty-three five-year-olds were selected from the ROLO Kids Study, a Randomized controlled trial Of a LOw glycemic index dietary intervention from the second trimester of pregnancy. DNA methylation was investigated in 780,501 CpG sites in DNA isolated from saliva. Principal component analysis identified no association between maternal age, weight, or body mass index (BMI) during pregnancy and offspring DNA methylation ( > 0.01). There was no association with the dietary intervention during pregnancy, however, gene pathway analysis identified functional clusters involved in insulin secretion and resistance that differed between the intervention and control. There were no associations with child weight or adiposity at five years of age; however, change in weight from six months was associated with variation in methylation. We identified no evidence of long-lasting influences of maternal diet or factors on DNA methylation at age five years. However, changes in child weight were associated with the methylome in childhood.
一系列宫内和生命早期的因素会影响后代的表观遗传学，特别是 DNA 甲基化模式。本研究旨在调查饮食干预和妊娠因素对 5 岁后代表观遗传特征的影响。我们还通过横断面分析探讨了身体成分与甲基化特征之间的关联。从 ROLO 儿童研究中选择了 63 名 5 岁儿童，该研究是一项从妊娠中期开始的低升糖指数饮食干预的随机对照试验。从唾液中分离出的 DNA 中检测了 780501 个 CpG 位点的 DNA 甲基化。主成分分析发现母亲年龄、怀孕期间的体重或体重指数（BMI）与后代的 DNA 甲基化之间没有关联（> 0.01）。怀孕期间的饮食干预也没有关联，但是基因途径分析确定了涉及胰岛素分泌和抵抗的功能群，它们在干预组和对照组之间存在差异。与 5 岁时儿童的体重或肥胖没有关联；然而，从 6 个月开始的体重变化与甲基化的变化有关。我们没有发现母亲饮食或因素对 5 岁时 DNA 甲基化有持久影响的证据。然而，儿童体重的变化与儿童时期的甲基组学有关。
