Hlophe Nqobile P, Aremu Adeyemi O, Doležal Karel, Staden Johannes Van, Finnie Jeffrey F
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
Indigenous Knowledge Systems (IKS) Centre, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2790, South Africa.
Plants (Basel). 2020 Nov 26;9(12):1657. doi: 10.3390/plants9121657.
In Africa and Asia, members of the genus are well-known for their diverse uses, especially their medicinal and nutritional values. However, the use of many species as a valuable resource is generally accompanied by the concern of over-exploitation attributed to their slow growth and general small size. The aim of the current study was to establish efficient micropropagation protocols for three species, namely (endangered), (vulnerable) and (least concern), as a means of ensuring their conservation and survival. This was achieved using nodal segments (~10 mm in length) as the source of explants in the presence of different concentrations of three cytokinins (CK) namely -benzyladenine (BA), isopentenyladenine (iP) and -topolin riboside (TR), over a period of 6 weeks. The highest (25 µM) concentration of cytokinin treatments typically resulted in significantly higher shoot proliferation. However, each species differed in its response to specific CK: the optimal concentrations were 25 µM TR, 25 µM iP and 25 µM BA for , and , respectively. During the in vitro propagation, both and rooted poorly while regenerated generally lacked roots regardless of the CK treatments. Following pulsing (dipping) treatment of in vitro-regenerated shoots with indole-3-butyric acid (IBA), acclimatization of all three species remained extremely limited due to poor rooting ex vitro. To the best of our knowledge, the current protocols provide the first successful report for these species. However, further research remains essential to enhance the efficiency of the devised protocol.
在非洲和亚洲，该属的成员因其多种用途而闻名，尤其是它们的药用和营养价值。然而，许多该属物种作为一种宝贵资源被利用的同时，人们普遍担心由于其生长缓慢和总体体型较小而被过度开发。本研究的目的是为三种该属物种建立高效的微繁殖方案，这三种物种分别是（濒危）、（易危）和（无危），以此作为确保它们得以保护和存活的一种手段。在6周的时间里，以节段（长度约10毫米）作为外植体来源，在存在三种不同浓度细胞分裂素（CK）的情况下进行，这三种细胞分裂素分别是6-苄基腺嘌呤（BA）、异戊烯基腺嘌呤（iP）和反式玉米素核苷（TR）。细胞分裂素处理的最高（25微摩尔）浓度通常导致显著更高的芽增殖。然而，每个物种对特定细胞分裂素的反应不同：对于、和，最佳浓度分别是25微摩尔TR、25微摩尔iP和25微摩尔BA。在离体繁殖过程中，和生根较差，而再生的无论细胞分裂素处理如何通常都缺乏根。在用吲哚-3-丁酸（IBA）对离体再生芽进行脉冲（浸泡）处理后，由于离体生根较差，所有三种该属物种的驯化仍然极其有限。据我们所知，目前的方案为这些该属物种提供了首个成功的报告。然而，进一步的研究对于提高所设计方案的效率仍然至关重要。
