Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215 India.
Biotechnol Lett. 2010 Sep;32(9):1199-205. doi: 10.1007/s10529-010-0290-0. Epub 2010 May 9.
The ultimate success of micropropagation on a commercial scale depends on the ability to transfer plants out of culture on a large scale, at low cost and with high survival rates. During field transfer the in vitro grown plantlets are unable to compete with soil microbes and to cope with the environmental conditions. The in vitro culture conditions result in the plantlets with altered morphology, anatomy and physiology. In order to increase growth and reduce mortality in plantlets at the acclimatisation stage, efforts are focused on the control of both physical and chemical environment and biohardening of micropropagated plantlets. This review describes the abiotic and biotic stresses and current developing methods for the acclimatization of microshoots.
大规模商业化微繁殖的最终成功取决于能否以低成本和高成活率将植物大量从培养中转移出来。在野外转移过程中，体外生长的组培苗无法与土壤微生物竞争，也无法适应环境条件。体外培养条件导致植物形态、解剖和生理发生改变。为了在驯化阶段增加组培苗的生长并降低死亡率，人们致力于控制物理和化学环境，并对微繁殖的组培苗进行生物强化。本文综述了非生物和生物胁迫以及目前用于微芽驯化的发展方法。
