Department of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Jeollabuk-do 55365, Korea.
Department of Food and Biotechnology, Korea University, Sejong 30019, Korea.
Nutrients. 2020 Nov 26;12(12):3640. doi: 10.3390/nu12123640.
Mustard leaf ( var. L. H. ) has been reported to have psychological properties such as anti-depressant activities. However, studies on chronic stress and depression caused by restraint have not been conducted. Therefore, this study aimed to evaluate the effects of a mustard leaf (ML) extract on chronic restraint stress (CRS) in mice. Male mice were subjected to a CRS protocol for a period of four weeks to induce stress. The results showed that the ML extract (100 and 500 mg/kg/perorally administered for four weeks) significantly decreased corticosterone levels and increased neurotransmitters levels in stressed mice. Apoptosis by CRS exposure was induced by and expression regulation and was suppressed by reducing and expression after treatment with the ML extract. Our results confirmed that apoptosis was regulated by increased expression of brain-derived neurotrophic factor (). Additionally, cytokine levels were regulated by the ML extract. In conclusion, our results showed that the ML extract relieved stress effects by regulating hormones and neurotransmitters in CRS mice, expression, and apoptosis in the brain. Thus, it can be suggested that the studied ML extract is an agonist that can help relieve stress and depression.
芥菜叶（ var. L. H. ）已被报道具有抗抑郁等心理特性。然而，关于束缚引起的慢性应激和抑郁的研究尚未进行。因此，本研究旨在评估芥菜叶（ML）提取物对小鼠慢性束缚应激（CRS）的影响。雄性小鼠接受 CRS 方案处理四周以诱发应激。结果表明，ML 提取物（100 和 500mg/kg/天，连续给药四周）可显著降低应激小鼠的皮质酮水平并增加神经递质水平。CRS 暴露诱导的细胞凋亡通过 和 表达调控，用 ML 提取物处理后， 通过降低 和 表达得到抑制。我们的结果证实，细胞凋亡通过脑源性神经营养因子（）的表达增加得到调节。此外，细胞因子水平也受到 ML 提取物的调节。总之，我们的结果表明，ML 提取物通过调节 CRS 小鼠的激素和神经递质、 表达和大脑中的细胞凋亡来缓解应激效应。因此，可以认为所研究的 ML 提取物是一种能够帮助缓解压力和抑郁的激动剂。
