从伊朗胡齐斯坦省和法尔斯省健康儿童的共生大肠杆菌分离株中发现的抗微生物耐药性和金属β-内酰胺酶产生情况。
Antimicrobial resistance and metallo-beta-lactamase producing among commensal Escherichia coli isolates from healthy children of Khuzestan and Fars provinces; Iran.
机构信息
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Postal code: 6135743135, Iran.
Mathematical Sciences and Computer Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
出版信息
BMC Microbiol. 2020 Dec 1;20(1):366. doi: 10.1186/s12866-020-02051-8.
BACKGROUND
The emergence of metallo-β-lactamase (MBL)-producing isolates is alarming since they carry mobile genetic elements with great ability to spread; therefore, early detection of these isolates, particularly their reservoir, is crucial to prevent their inter- and intra-care setting dissemination and establish suitable antimicrobial therapies. The current study was designed to evaluate the frequency of antimicrobial resistance (AMR), MBL producers and identification of MBL resistance genes in Escherichia coli strains isolated from fecal samples of the healthy children under 3 years old. A total of 412 fecal E. coli isolates were collected from October 2017 to December 2018. The study population included healthy infants and children aged < 3 years who did not exhibit symptoms of any diseases, especially gastrointestinal diseases. E. coli isolates were assessed to determine the pattern of AMR. E. coli isolates were assessed to determine the pattern of AMR, the production of extended spectrum β-lactamase (ESBL) and MBL by phenotypic methods. Carbapenem-resistant isolates were investigated for the presence of MBL and carbapenemase genes, plasmid profiling, and the ability of conjugation.
RESULTS
In sum, AMR, multi-drug resistance (MDR) and ESBL production were observed in more than 54.9, 36.2 and 11.7% of commensal E. coli isolates, respectively. Out of six isolates resistant to imipenem and meropenem, four isolates were phenotypically detected as MBL producers. Two and one E. coli strains carried the bla and bla genes, respectively and were able to transmit imipenem resistance through conjugation.
CONCLUSION
Our findings showed that children not exposed to antibiotics can be colonized by E. coli isolates resistant to the commonly used antimicrobial compounds and can be a good indicator for the occurrence and prevalence of AMR in the community. These bacteria can act as a potential reservoir of AMR genes including MBL genes of pathogenic bacteria and lead to the dissemination of resistance mechanisms to other bacteria.
背景
产金属β-内酰胺酶（MBL）的分离株的出现令人警惕，因为它们带有具有强大传播能力的移动遗传元件；因此，早期检测这些分离株，特别是它们的储库，对于防止它们在不同医疗环境中传播以及建立合适的抗菌治疗方法至关重要。本研究旨在评估 3 岁以下健康儿童粪便样本中分离的大肠埃希菌的抗菌药物耐药（AMR）、MBL 产生情况及其 MBL 耐药基因的鉴定。2017 年 10 月至 2018 年 12 月期间共收集了 412 株粪便大肠埃希菌。研究人群包括未表现出任何疾病，尤其是胃肠道疾病症状的健康婴儿和 3 岁以下儿童。评估大肠埃希菌分离株以确定 AMR 模式。通过表型方法评估大肠埃希菌分离株对 AMR、超广谱β-内酰胺酶（ESBL）和 MBL 的产生。对耐碳青霉烯的分离株进行 MBL 和碳青霉烯酶基因检测、质粒谱分析和接合能力研究。
结果
在 412 株粪便大肠埃希菌中，超过 54.9%、36.2%和 11.7%的分离株表现出 AMR、多药耐药（MDR）和 ESBL 产生。6 株对亚胺培南和美罗培南耐药的分离株中，有 4 株被表型检测为 MBL 产生菌。有 2 株和 1 株大肠埃希菌携带 bla 和 bla 基因，能够通过接合传递对亚胺培南的耐药性。
结论
我们的研究结果表明，未接触过抗生素的儿童可能会被对常用抗菌化合物耐药的大肠埃希菌分离株定植，并可能成为社区中 AMR 发生和流行的良好指标。这些细菌可能成为包括 MBL 基因在内的致病性耐药基因的潜在储库，并导致耐药机制向其他细菌传播。
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