Department of Microbiology and Infectious Diseases, Liverpool Hospital, Liverpool, New South Wales, Australia
Department of Microbiology and Infectious Diseases, Liverpool Hospital, Liverpool, New South Wales, Australia.
J Clin Microbiol. 2019 Jun 25;57(7). doi: 10.1128/JCM.01852-18. Print 2019 Jul.
Accurate and timely identification of carbapenemase-producing (CPE) is critical for microbiology laboratories in order to facilitate infection prevention, reduce the spread of multiresistant Gram-negative bacilli, and reduce delays to effective antibiotic therapy. We undertook a study to compare the carbapenem inactivation method (CIM) against the modified carbapenem inactivation method (mCIM) on a broad range of CPE isolates from Australia, including a high proportion of isolates. Furthermore, we evaluated the performance of the mCIM with a reduced incubation time using automated incubation and digital plate reading in order to better facilitate quick confirmation of carbapenemases. The overall sensitivity of the mCIM was 98.2%, compared to 95.6% for the CIM. The minimum incubation time for the mCIM while maintaining its sensitivity was 12 hours. Both the CIM and mCIM perform well on a broad range of CPE isolates seen in Australia.
准确且及时地鉴定碳青霉烯酶产生菌（CPE）对于微生物学实验室至关重要，这有助于预防感染、减少耐药革兰氏阴性杆菌的传播，并减少抗生素治疗的延误。我们进行了一项研究，比较了碳青霉烯失活法（CIM）和改良碳青霉烯失活法（mCIM）在澳大利亚广泛的 CPE 分离株中的表现，其中包括很高比例的 分离株。此外，我们使用自动孵育和数字平板读数评估了缩短孵育时间的 mCIM 的性能，以便更方便地快速确认碳青霉烯酶。mCIM 的总灵敏度为 98.2%，而 CIM 的灵敏度为 95.6%。mCIM 保持其灵敏度的最短孵育时间为 12 小时。CIM 和 mCIM 均可很好地检测到澳大利亚广泛存在的 CPE 分离株。
