Department of Laboratory Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing, China.
Birth Defects Prevention and Control Technology Research Center, Chinese PLA General Hospital, Beijing, China.
Front Cell Infect Microbiol. 2021 Dec 13;11:743390. doi: 10.3389/fcimb.2021.743390. eCollection 2021.
Faecal can act as reservoirs for resistance genes. Here, we analyzed prevalence of drug resistance in faecal isolated from healthy children at a single kindergarten in Beijing, China, then used whole genome sequencing to characterize fluoroquinolone-non-susceptible strains. Our results revealed high resistance to ampicillin (54.0%), trimethoprim/sulphurmethoxazole (47.5%) and tetracycline (58.9%) among 576 faecal isolates, 49.2% of which exhibited multidrug resistance. A total of 113 isolates were not susceptible to ciprofloxacin, with four sequence types, namely ST1193 (25.7%), ST773 (13.3%), ST648 (8.8%) and ST131 (7.1%) found to be the most prevalent (54.9%). With regards to resistance to quinolones, we detected chromosomal mutations in , , and in 111 (98.2%), 105 (92.9%), and 67 (61.1%) isolates, respectively. (37.2%) was the major ESBL gene, whereas (12.4%) and (11.5%) were the most frequent subtypes. A total of 90 (79.6%) ExPEC and 65 (57.5%) UPEC isolates were classified. Overall, these findings revealed clonal spread of certain prevalent STs, namely ST1193, ST773, ST648 and ST131 isolates in healthy children within a single kindergarten in Beijing, China, affirming the seriousness of the multidrug resistance problem and potential pathogenicity of isolates in healthy children. Therefore, there is an urgent need for increased surveillance to enhance control of this problem.
粪便可能成为耐药基因的储存库。在这里，我们分析了中国北京一所幼儿园健康儿童粪便中分离的耐药基因的流行情况，然后使用全基因组测序对氟喹诺酮类药物不敏感株进行特征分析。我们的结果显示，576 株粪便分离株中氨苄西林（54.0%）、复方磺胺甲噁唑（47.5%）和四环素（58.9%）耐药率较高，其中 49.2%表现为多重耐药。共有 113 株对环丙沙星不敏感，有 4 种序列类型，即 ST1193（25.7%）、ST773（13.3%）、ST648（8.8%）和 ST131（7.1%），发现最普遍（54.9%）。关于对喹诺酮类药物的耐药性，我们在 111（98.2%）、105（92.9%）和 67（61.1%）分离株中分别检测到 、 和 中的染色体突变。 （37.2%）是主要的 ESBL 基因，而 （12.4%）和 （11.5%）是最常见的亚型。共有 90（79.6%）种 ExPEC 和 65（57.5%）种 UPEC 分离株被分类。总的来说，这些发现表明，某些流行的 STs（如 ST1193、ST773、ST648 和 ST131）在中国北京的一所幼儿园内的健康儿童中发生了克隆传播，证实了健康儿童中 分离株的多药耐药问题和潜在致病性的严重性。因此，迫切需要加强监测，以加强对此问题的控制。
