Wadsworth Center, New York State Department of Health, Albany, New York, USA.
School of Biological Sciences, University of Utah, Salt Lake City, Utah, USA.
J Bacteriol. 2021 Jan 25;203(4). doi: 10.1128/JB.00510-20.
FliA is a broadly conserved σ factor that directs transcription of genes involved in flagellar motility. We previously identified FliA-transcribed genes in and serovar Typhimurium, and we showed that FliA transcribes many unstable, noncoding RNAs from intragenic promoters. Here, we show that FliA in Typhimurium also directs the transcription of large numbers of unstable, noncoding RNAs from intragenic promoters, and we identify two previously unreported FliA-transcribed protein-coding genes. One of these genes, , encodes a transcription factor that responds to quorum-sensing signals produced by other bacteria. We show that FliA-dependent transcription of is required for SdiA activity, highlighting a regulatory link between flagellar motility and intercellular communication. Initiation of bacterial transcription requires association of a σ factor with the core RNA polymerase to facilitate sequence-specific recognition of promoter elements. FliA is a widely conserved σ factor that directs transcription of genes involved in flagellar motility. We previously showed that FliA transcribes many unstable, noncoding RNAs from promoters within genes. Here, we demonstrate the same phenomenon in Typhimurium. We also show that Typhimurium FliA directs transcription of the gene, which encodes a transcription factor that responds to quorum-sensing signals produced by other bacteria. FliA-dependent transcription of is required for transcriptional control of SdiA target genes, highlighting a regulatory link between flagellar motility and intercellular communication.
FliA 是一种广泛保守的 σ 因子，可指导鞭毛运动相关基因的转录。我们之前在 和 血清型鼠伤寒沙门氏菌中鉴定了 FliA 转录的基因，并表明 FliA 从基因内启动子转录许多不稳定的非编码 RNA。在这里，我们表明 鼠伤寒沙门氏菌中的 FliA 也指导大量不稳定的、来自基因内启动子的非编码 RNA 的转录，并且我们鉴定了两个以前未报道的 FliA 转录的蛋白编码基因。其中一个基因， ，编码一个转录因子，该转录因子响应其他细菌产生的群体感应信号。我们表明，SdiA 活性需要 FliA 依赖性的 转录，这突出了鞭毛运动和细胞间通讯之间的调节联系。细菌转录的起始需要 σ 因子与核心 RNA 聚合酶结合，以促进启动子元件的序列特异性识别。FliA 是一种广泛保守的 σ 因子，可指导鞭毛运动相关基因的转录。我们之前表明， FliA 从基因内启动子转录许多不稳定的非编码 RNA。在这里，我们在 鼠伤寒沙门氏菌中证明了同样的现象。我们还表明， 鼠伤寒沙门氏菌 FliA 指导 基因的转录，该基因编码一个转录因子，该转录因子响应其他细菌产生的群体感应信号。 转录需要 FliA 依赖性转录，以实现 SdiA 靶基因的转录调控，这突出了鞭毛运动和细胞间通讯之间的调节联系。
