College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Division of Polar Life Science, Korea Polar Research Institute, Incheon 21990, Republic of Korea.
Zool Res. 2021 Jan 18;42(1):124-129. doi: 10.24272/j.issn.2095-8137.2020.264.
The Antarctic toothfish, , belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of , which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.
南极犬牙鱼属于南极鱼科，分布在南纬 S60°以下的低温区域的南大洋中。因此，它是研究冷适应特征状态的一个有吸引力的模式物种。在本研究中，我们成功生成了南极犬牙鱼的高连续基因组序列，该序列包含 1 062 个支架，N50 长度为 36.98Mb，最长支架长度为 46.82Mb。重复元件占基因组的 40.87%。我们还使用基因预测和转录组测序推断了 32914 个蛋白质编码基因，并使用 Isoform-Sequencing（Iso-Seq）检测了剪接变体，这将是进一步探索南极犬牙鱼适应机制的宝贵资源。这个南极犬牙鱼的新高质量参考基因组为更深入地了解冷适应和物种保护提供了基础资源。
