Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea.
2National Institute of Fisheries Science (NIFS), Busan, 46083, Korea.
Sci Data. 2024 Nov 16;11(1):1240. doi: 10.1038/s41597-024-04119-w.
The Patagonian toothfish (Dissostichus eleginoides) belongs to the Actinopterygii class, and the suborder Notothenioidei, which lives in cold waters in the Southern Hemisphere. We performed assembly and annotation, and we integrated the Illumina short-read sequencing for polishinng, PacBio long-read sequencing for contig-level assembly, and Hi-C sequencing technology to obtain high-quality of chromosome-level genome assembly. The final assembly analysis resulted in a total of 495 scaffolds, a genome size of 844.7 Mbp and an N50 length of 36 Mbp. Among these data, we confirmed 24 scaffolds exceeded 10 Mbp and classified as chromosome-level. The completeness of BUSCO rate was over 97%. A total gene set of 32,224 was identified. Furthermore, we analyzed the presence of AFGP genes, classified into Antarctic and sub-Antarctic categories through phylogenetic analysis. This study provides a useful resource for the genomic analysis of Patagonian toothfish and genetic insights into the comparison with Antarctic fishes.
巴塔哥尼亚牙鱼（Dissostichus eleginoides）属于硬骨鱼纲，南极鱼亚目，生活在南半球的冷水域。我们进行了组装和注释，并整合了 Illumina 短读测序进行抛光、PacBio 长读测序进行拼接级组装，以及 Hi-C 测序技术以获得高质量的染色体水平基因组组装。最终的组装分析共得到 495 个支架，基因组大小为 844.7 Mbp，N50 长度为 36 Mbp。在这些数据中，我们确认了 24 个支架超过 10 Mbp，并归类为染色体水平。BUSCO 率的完整性超过 97%。总共鉴定出 32224 个基因。此外，我们通过系统发育分析分析了 AFGP 基因的存在，将其分为南极和亚南极两类。本研究为巴塔哥尼亚牙鱼的基因组分析提供了有用的资源，并为与南极鱼类的遗传比较提供了遗传见解。
