Department of Chemistry, Tokat Gaziosmanpaşa University, 60250 Tokat, Turkey.
Department of Chemistry, University of Turbat, Balochistan, Pakistan.
J AOAC Int. 2021 May 21;104(2):384-388. doi: 10.1093/jaoacint/qsaa107.
A new and simple effervescent CO2-table-induced switchable hydrophilicity solvent extraction method (CI-SHS) was used for the preconcentration of rhodamine B (RB).
The main theme of the proposed method to disperse green solvent by in situ CO2-table system overcome on the contamination and to avoid the dispersion by using different chemicals.
Switchable hydrophilicity solvent (N, N-Dimethyl cyclohexylamine) was dispersed in an aqueous solution by reaction of CO2-table of sodium carbonate and citric acid to enhance the contact area between two medium, which radically improved the extraction capacity of the RB from aqueous medium to switchable solvent. The phase separation was achieved by simple heating not by centrifugation that makes a change on hydrophilicity to hydrophobicity. The supernatant-enriched RB- N, N-Dimethyl cyclohexylamine phase was withdrawn and injected into the UV glass cells. The absorbance was measured at 542.
Limit of detection and enhancement factor were achieved to be 0.042 and 50 µg/L. Validity and accuracy of the method verified by analysis of real samples by the spiking addition method. The relative standard deviation was obtained at 3.2%. The method was applied to food and soft drink samples.
This method offers high sensitivity, selectivity, accuracy, and precision for the separation, preconcentration, and spectrophotometric determination of RB. Simple, fast, low-cost, and green extraction method was developed.
High enrichment factor and lower detection limits were observed in this microextraction method. Present method is sensitive, selective, very accurate, and precise.
一种新的、简单的 CO2 -table 诱导的可切换亲水性溶剂萃取方法（CI-SHS）被用于罗丹明 B（RB）的预浓缩。
所提出的方法的主要主题是通过原位 CO2-table 系统分散绿色溶剂，克服污染问题，并避免使用不同化学物质进行分散。
可切换亲水性溶剂（N，N-二甲基环己胺）通过碳酸钠和柠檬酸的 CO2-table 反应分散在水溶液中，以增强两种介质之间的接触面积，从而极大地提高了 RB 从水介质到可切换溶剂的萃取能力。通过简单的加热而不是离心来实现相分离，这使得亲水性发生变化。将富含 RB-N，N-二甲基环己胺相的上清液抽出并注入到 UV 玻璃池中。在 542nm 处测量吸光度。
检测限和增强因子分别达到 0.042 和 50µg/L。通过添加标准法对实际样品进行分析验证了该方法的有效性和准确性。相对标准偏差为 3.2%。该方法已应用于食品和软饮料样品的分析。
该方法为 RB 的分离、预浓缩和分光光度法测定提供了高灵敏度、选择性、准确性和精密度。开发了一种简单、快速、低成本和绿色的萃取方法。
在这种微萃取方法中观察到高的富集因子和低的检测限。本方法具有灵敏度高、选择性好、准确性和精密度高等特点。
