Sivas Cumhuriyet University, Faculty of Science, Department of Chemistry, Sivas, Turkey.
Sivas Cumhuriyet University, Faculty of Science, Department of Chemistry, Sivas, Turkey.
Food Chem. 2023 Jan 15;399:133929. doi: 10.1016/j.foodchem.2022.133929. Epub 2022 Aug 11.
The main purpose of this research article is to develop a vortex-assisted switchable hydrophilicity solvent liquid phase microextraction (VA-SHS-LPME) for the selective and efficient extraction of trace vanillin in food samples. Four switchable hydrophilicity solvents (SHSs) were prepared and tested for the extraction of vanillin. The obtained extract phase after phase separation was analyzed by UV-vis spectrophotometry. The extraction parameters including pH, vortex time, NaOH volume, and SHS volume were optimized using central composite design based on response surface methodology. Under optimized conditions, the linear range (0.2-400 ng mL with r = 0.9985), limit of detection (0.06 ng mL), limit of quantitation (2.0 ng mL), extraction recovery (97 ± 4 %) and enhancement factor (220) were obtained. Also, relative standard deviations were less than 2.1 % indicating good precision. The VA-SHS-LPME procedure showed some advantages including good extraction, low consumption of chemical and low matrix effect. Finally, the VA-SHS-LPME procedure was applied for the determination of vanillin in food samples, and acceptable recoveries (91 ± 3-99 ± 3 %) were obtained.
本研究文章的主要目的是开发一种涡旋辅助切换亲水性溶剂液相微萃取（VA-SHS-LPME）方法，用于选择性和高效提取食品样品中的痕量香草醛。本文制备并测试了四种切换亲水性溶剂（SHS）以萃取香草醛。通过紫外可见分光光度法对相分离后得到的萃取相进行分析。通过基于响应面法的中心复合设计，优化了包括 pH、涡旋时间、NaOH 体积和 SHS 体积在内的萃取参数。在优化条件下，该方法的线性范围为 0.2-400ngmL，r=0.9985，检测限为 0.06ngmL，定量限为 2.0ngmL，萃取回收率为 97±4%，增强因子为 220。相对标准偏差均小于 2.1%，表明具有良好的精密度。VA-SHS-LPME 方法具有良好的萃取效果、低化学试剂消耗和低基质效应等优点。最后，将 VA-SHS-LPME 方法应用于食品样品中香草醛的测定，获得了可接受的回收率（91±3-99±3%）。
