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[一种评估胰岛胰岛素水平的组织荧光测定法]

[A histofluorimetric method of evaluating the insulin level of pancreatic islets].

作者信息

Meĭramov G G, Tusupbekova G T, Meĭramova R G

出版信息

Probl Endokrinol (Mosk). 1987 Nov-Dec;33(6):49-51.

PMID:3325972
Abstract

Up to now results of the histochemical luminiscent reaction to insulin in pancreatic islet beta cells (the pseudoisocyanin test) were assessed by a visual method which permitted establishing the difference only in substantial variations of fluorescence intensity. The authors employed a quantitative method for assessment of the results of the test using a microfluorimetric attachment designed by them. They also provided data on the measurement of the insulin content in pancreatic islet beta cells of rats in different experimental exposures using this method.

摘要

到目前为止,胰岛β细胞中胰岛素的组织化学发光反应(假异氰蓝试验)结果是通过视觉方法评估的,该方法仅能在荧光强度有显著变化时确定差异。作者采用了一种定量方法来评估试验结果,使用的是他们自己设计的微量荧光测定附件。他们还提供了使用该方法测量不同实验条件下大鼠胰岛β细胞中胰岛素含量的数据。

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