Cellular and Molecular Sciences Group, James Hutton Institute, Dundee DD2 5DA, UK.
School of Biology, University of St Andrews, North Haugh, St Andrews KY16 9TZ, UK.
Genes (Basel). 2020 Nov 28;11(12):1429. doi: 10.3390/genes11121429.
Although the use of natural resistance is the most effective management approach against the potato cyst nematode (PCN) , the existence of pathotypes with different virulence characteristics constitutes a constraint towards this goal. Two resistance sources, (from ) and from ssp. CPC2802 (from the Commonwealth Potato Collection) are widely used in potato breeding programmes in European potato industry. However, the use of resistant cultivars may drive strong selection towards virulence, which allows the increase in frequency of virulent alleles in the population and therefore, the emergence of highly virulent nematode lineages. This study aimed to identify () genes in populations selected for virulence on the above resistance sources, and the genomic impact of selection processes on the nematode. The selection drive in the populations was found to be specific to their genetic background. At the genomic level, 11 genes were found that represent candidate genes. Most of the variant calls determining selection were associated with -selected populations, while many of them seem to be organised in genomic islands facilitating selection evolution. These phenotypic and genomic findings combined with histological studies performed revealed potential mechanisms underlying selection in .
虽然利用天然抗性是防治马铃薯胞囊线虫（PCN）最有效的管理方法，但存在具有不同毒力特征的菌系是实现这一目标的一个限制因素。两种抗性来源， （来自）和 来自 ssp. CPC2802（来自英联邦马铃薯收藏）广泛用于欧洲马铃薯产业的马铃薯育种计划。然而，抗性品种的使用可能会强烈地选择毒力，这允许在种群中增加毒力等位基因的频率，从而导致高度毒力线虫谱系的出现。本研究旨在鉴定在上述抗性来源上选择毒力的 种群中的 ()基因，以及选择过程对线虫的基因组影响。种群中的选择驱动力被发现与其遗传背景特异性相关。在基因组水平上，发现了 11 个代表候选 基因的基因。确定选择的大多数变异调用与 -选择的种群有关，而其中许多似乎组织在有利于选择进化的基因组岛屿中。这些表型和基因组研究结果与进行的组织学研究相结合，揭示了 中选择的潜在机制。
