聚合酶 Pol ι 的链置换活性。
Strand Displacement Activity of PrimPol.
机构信息
Institute of Molecular Genetics, National Research Center "Kurchatov Institute", Kurchatov sq. 2, 123182 Moscow, Russia.
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090 Novosibirsk, Russia.
出版信息
Int J Mol Sci. 2020 Nov 27;21(23):9027. doi: 10.3390/ijms21239027.
Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5'-DNA flaps generated by PrimPol in vitro.
人源 PrimPol 是一种独特的酶，具有 DNA/RNA 引发酶和 DNA 聚合酶活性。在这项工作中，我们证明了 PrimPol 能够有效地填补 5 个核苷酸的缺口，并具有由 Mn 离子和辅助复制蛋白 RPA 和 PolDIP2 刺激的条件性链置换活性。PrimPol 的 DNA 置换活性比 RNA 置换活性更有效，并且 FEN1 处理了体外由 PrimPol 产生的 5'-DNA 发夹。
Zotero 插件