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[从细胞培养物中鉴定支原体分离株的实验]

[Experiments to identify Mycoplasma isolates from cell cultures].

作者信息

Petkova K, Dilovski M, Bŭchvarova Ia

出版信息

Vet Med Nauki. 1987;24(8):13-6.

PMID:3326249
Abstract

Identified were various species of Mycoplasma organisms isolated from three continuous cell lines--BHK-21, SPZF, and TT. On the base of cultural, biochemical, and serological investigations the isolates were defined as M. bovirhinis, M. arginini, and A. laidlawii. The bovine sera, used to culture the cell lines were shown to be the basic source of contamination with Mycoplasma organisms. This made it necessary to carry out a preliminary study on each batch of serum for Mycoplasma contamination prior to its use for laboratory and productional needs.

摘要

从三种连续细胞系——BHK - 21、SPZF和TT中分离出了各种支原体微生物。基于培养、生化和血清学研究,这些分离株被鉴定为牛鼻支原体、精氨酸支原体和莱氏无胆甾原体。用于培养细胞系的牛血清被证明是支原体微生物污染的主要来源。这使得有必要在将每批血清用于实验室和生产需求之前,对其支原体污染情况进行初步研究。

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