Department of Otolaryngology-Head & Neck Surgery, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
Future Oncol. 2021 Jan;17(1):57-69. doi: 10.2217/fon-2020-0618. Epub 2020 Dec 2.
To investigate the role of in nasopharyngeal carcinoma (NPC). Using NPC cells CNE-2 and HNE-2 , we performed quantitative PCR to determine mRNA expression and western blotting to determine protein expression. CCK-8, transwell, flow cytometry and wound healing assays were done to examine the function of and STAT1. Chromatin immunoprecipitation PCR (ChIP-PCR) confirmed that STAT1 combines with the promoter region. Xenograft experiments were used to verify the role of STAT1 and LINC01160 . is upregulated in NPC and can promote a malignant cell phenotype. STAT1 is a transcription factor of and can promote a malignant cell phenotype through upregulating expression. STAT1 can promote a malignant cell phenotype by upregulating .
研究 在鼻咽癌（NPC）中的作用。使用 NPC 细胞 CNE-2 和 HNE-2，我们进行了定量 PCR 以确定 mRNA 表达，进行了 Western blot 以确定蛋白表达。通过 CCK-8、Transwell、流式细胞术和划痕愈合实验研究了 的功能和 STAT1。染色质免疫沉淀 PCR（ChIP-PCR）证实 STAT1 与 启动子区域结合。通过异种移植实验验证了 STAT1 和 LINC01160 的作用。 在 NPC 中上调，并能促进恶性细胞表型。STAT1 是 的转录因子，可通过上调 表达促进恶性细胞表型。STAT1 可通过上调 促进恶性细胞表型。
