Hawley C E, Falkler W A
Infect Immun. 1977 Oct;18(1):124-9. doi: 10.1128/iai.18.1.124-129.1977.
Fusobacterium polymorphum has been isolated from the gingival crevice in humans and has been implicated in the immunopathology of periodontal diseases. The presence of alternative complement pathway factors in gingival crevice material suggests the contribution of this process in the manifestation of the local host response. A complement consumption assay was used to show that the anticomplementary activity of a cell-wall preparation from F. polymorphum in guinea pig complement progressed optimally at 37 degrees C and suboptimally at 0 degrees C. Similar levels of complement consumption were recorded at both temperatures, but a higher concentration of cell walls was required to demonstrate maximum activity in the cold. Augmentation experiments with additional normal and heat-treated complement showed the presence of heat-labile factors that controlled and limited the consumption of complement to a constant proportion of the total amount present. These same experiments indicated that there were no reactive antibodies in the complement used in the assay. Similar tests with C4-deficient guinea pig sera confirmed that F. polymorphum cell walls were capable of generating alternative complement pathway activity in guinea pig sera.
多形梭杆菌已从人类牙龈沟中分离出来，并与牙周疾病的免疫病理学有关。牙龈沟物质中替代补体途径因子的存在表明该过程在局部宿主反应表现中的作用。采用补体消耗试验表明，多形梭杆菌细胞壁制剂对豚鼠补体的抗补体活性在37℃时最佳，在0℃时次佳。两个温度下记录的补体消耗水平相似，但在低温下需要更高浓度的细胞壁才能显示最大活性。用额外的正常和热处理补体进行的增强实验表明存在热不稳定因子，这些因子将补体的消耗控制并限制在总量的恒定比例。这些相同的实验表明，试验中使用的补体中没有反应性抗体。用C4缺陷豚鼠血清进行的类似试验证实，多形梭杆菌细胞壁能够在豚鼠血清中产生替代补体途径活性。
