Zhao Qiyue, Li Han, Chen Hui, Wu Chao, Ei-Seedi Hesham, Xu Xianbing, Du Ming
National Engineering Research Center of Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-Construction for Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, People's Republic of China.
Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Uppsala, Sweden.
J Hazard Mater. 2021 Feb 5;403:123580. doi: 10.1016/j.jhazmat.2020.123580. Epub 2020 Aug 1.
Advanced analytical platforms are required for accurate detection and quantification of small molecular substances exhibiting certain toxicity. Small molecules detection in complex biological fluids are challenged by the complexity of the samples and the low throughput of the existing methods. In the present study, to detect a batch of samples (50) in 1 h, the plasmonic nanoshell enhanced matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) was tested. The limit of quantification (LOQ) was determined as 0.01 μg/mL (for α-dicarbonyl compounds) by vortex-assisted liquid-liquid microextraction (VALLME). The developed method can be adopted to study the high-throughput metabolomics and employed for clinical precision diagnosis with MALDI-TOF MS.
对于准确检测和定量具有一定毒性的小分子物质，需要先进的分析平台。复杂生物流体中的小分子检测受到样品复杂性和现有方法低通量的挑战。在本研究中，为了在1小时内检测一批样本（50个），对等离子体纳米壳增强基质辅助激光解吸/电离质谱（MALDI-TOF MS）进行了测试。通过涡旋辅助液-液微萃取（VALLME）测定定量限（LOQ）为0.01μg/mL（对于α-二羰基化合物）。所开发的方法可用于研究高通量代谢组学，并用于MALDI-TOF MS的临床精准诊断。
