Suo Tiying, Sohail Muhammad, Xie Siying, Li Bingzhi, Chen Yue, Zhang Lihui, Zhang Xing
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.
J Hazard Mater. 2021 Feb 5;403:123418. doi: 10.1016/j.jhazmat.2020.123418. Epub 2020 Jul 8.
The Microcystin-Leucine-Arginine (MC-LR) is the most toxic and widely distributed microcystin, which originates from cyanobacteria produced by water eutrophication. The MC-LR has deleterious effects on the aquatic lives and agriculture, and this highly toxic chemical could severely endanger human health when the polluted food was intaken. Therefore, the monitoring of MC-LR is of vital importance in the fields including environment, food, and public health. Utilizing the complementary base pairing between DNA molecules, DNA nanotechnology can realize the programmable and predictable regulation of DNA molecules. In analytical applications, DNA nanotechnology can be used to detect targets via target-induced conformation change and the nano-assemblies of nucleic acids. Compared with the conventional analytical technologies, DNA nanotechnology has the advantages of sensitive, versatile, and high potential in real-time and on-site applications. According to the molecular basis for recognizing MC-LR, the strategies of applying DNA nanotechnology in the MC-LR monitoring are divided into two categories in this review: DNA as a recognition element and DNA-assisted signal processing. This paper introduces state-of-the-art analytical methods for the detection of MC-LR based on DNA nanotechnology and provides critical perspectives on the challenges and development in this field.
微囊藻毒素 - 亮氨酸 - 精氨酸（MC - LR）是毒性最强且分布最广的微囊藻毒素，它源自水体富营养化产生的蓝藻。MC - LR对水生生物和农业有有害影响，当摄入受污染的食物时，这种剧毒化学物质会严重危及人类健康。因此，MC - LR的监测在环境、食品和公共卫生等领域至关重要。利用DNA分子之间的互补碱基配对，DNA纳米技术可以实现对DNA分子的可编程和可预测调控。在分析应用中，DNA纳米技术可通过靶标诱导的构象变化和核酸的纳米组装来检测靶标。与传统分析技术相比，DNA纳米技术具有灵敏、通用以及在实时和现场应用方面潜力巨大的优点。根据识别MC - LR的分子基础，本综述将DNA纳米技术应用于MC - LR监测的策略分为两类：DNA作为识别元件和DNA辅助信号处理。本文介绍了基于DNA纳米技术检测MC - LR的最新分析方法，并对该领域的挑战和发展提供了关键见解。
