Entropic Stabilization of Cas4 Protein SSO0001 Predicted with Popcoen.

is a method for configurational entropy estimation of proteins based on machine-learning. Entropy is predicted with an artificial neural network which was trained on simulation trajectories of a large set of representative proteins. is extremely fast compared to other approaches based on the sampling of a multitude of microstates. Consequently, can be incorporated into a large class of protein software which currently neglects configurational entropy for performance reasons. Here, we apply to various conformations of the Cas4 protein SSO0001 of , a protein that assembles to a decamer of known toroidal shape. We provide numerical evidence that the native state (NAT) of a SSO0001 monomer has a similar structure to the protomers of the oligomer, where NAT of the monomer is stabilized mainly entropically. Due to its large amount of configurational entropy, NAT has lower free energy than alternative conformations of very low enthalpy and solvation free-energy. Hence, SSO0001 serves as an example case where neglecting configurational entropy leads to incorrect conclusion. Our results imply that no refolding of the subunits is required during oligomerization which suggests that configurational entropy is employed by nature to largely enhance the rate of assembly.