Abe J, Okamura H, Makino S, Yanaihara N, Ibata Y
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Brain Res Bull. 1987 Dec;19(6):735-41. doi: 10.1016/0361-9230(87)90061-x.
Recently, [Met]Enkephalin-Arg-Gly-Leu (MEAGL) was isolated from bovine adrenal glands, and it was found to be derived exclusively from proenkephalin. Therefore, we investigated the distribution of MEAGL-like immunoreactive neuronal perikarya and fibers in the rat diencephalon pretreated with colchicine by PAP immunocytochemistry. In the thalamus MEAGL immunoreactive neuronal perikarya were distributed in the paraventricular nucleus and the ventral part of the lateral geniculate nucleus. Immunoreactive fibers were found in the paraventricular, paracentral, anteroventral, reuniens and rhomboid nuclei. In addition, immunoreactive fibers were also noted in the anterior pretectal nucleus. In the hypothalamus, immunoreactive neuronal perikarya were observed in the medial preoptic area, anterior and lateral hypothalamic nuclei, perifornical region, parvocellular and postero-magnocellular regions of paraventricular nucleus, ventromedial nucleus, dorsomedial nucleus, arcuate nucleus, premammillary, medial mammillary and lateral mammillary nuclei. The distribution of immunoreactive fibers was similar to that of neuronal perikarya. However, immunoreactive fibers were also observed in the supraoptic and suprachiasmatic nuclei where no immunoreactive neuronal perikarya were detected. Numerous immunoreactive fibers were detected in the external layer of the median eminence, but there were few in the internal layer. The similarity and difference in the distribution between MEAGL and other proenkephalin peptides such as [Met]enkephalin were also discussed.
最近，[甲硫氨酸]脑啡肽-精氨酸-甘氨酸-亮氨酸（MEAGL）从牛肾上腺中分离出来，并且发现它仅来源于前脑啡肽。因此，我们通过过氧化物酶抗过氧化物酶免疫细胞化学方法研究了用秋水仙碱预处理的大鼠间脑中MEAGL样免疫反应性神经元胞体和纤维的分布。在丘脑中，MEAGL免疫反应性神经元胞体分布在室旁核和外侧膝状体核的腹侧部分。在室旁核、中央旁核、前腹核、连合核和菱形核中发现了免疫反应性纤维。此外，在前顶盖前核中也注意到了免疫反应性纤维。在下丘脑中，在内侧视前区、下丘脑前核和外侧核、穹窿周区、室旁核的小细胞和后大细胞区、腹内侧核、背内侧核、弓状核、乳头前核、内侧乳头核和外侧乳头核中观察到了免疫反应性神经元胞体。免疫反应性纤维的分布与神经元胞体相似。然而，在视上核和视交叉上核中也观察到了免疫反应性纤维，而在这些核中未检测到免疫反应性神经元胞体。在正中隆起的外层检测到大量免疫反应性纤维，但在内层则很少。还讨论了MEAGL与其他前脑啡肽肽如[甲硫氨酸]脑啡肽在分布上的异同。
