School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha, China.
School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha, China.
Food Chem. 2021 Apr 16;342:128352. doi: 10.1016/j.foodchem.2020.128352. Epub 2020 Oct 19.
Rice bran dietary fiber (ERBDF) subjected to pre-water-washing and complex enzyme treatment using heat-stable α-amylase, alcalase, and glucoamylase had significantly higher (p < 0.05) proportions of cellulose, hemicellulose, lignin, and lower proportions of lipid, protein, and starch than rice bran dietary fiber subjected to complex enzyme treatment without pre-water-washing. Cellulase modification of ERBDF significantly decreased (p < 0.05) cellulose, hemicellulose, starch, and protein contents while the relative lignin content increased. Cellulase modification significantly improved (p < 0.05) water-holding capacity, oil-holding capacity, swelling capacity, cholesterol absorption capacity, and glucose adsorption capacity, while decreasing the emulsifying capacity and glucose dialysis retardation index. The changes of physicochemical and functional properties of fiber samples after cellulase modification were attributed to the increased porosity of the fiber surface, greater exposure of binding sites caused by reduced crystallinity, and changes to the chemical composition.
经预水洗和使用热稳定α-淀粉酶、碱性蛋白酶和糖化酶的复合酶处理后的米糠膳食纤维(ERBDF),其纤维素、半纤维素、木质素的比例显著高于未经预水洗的复合酶处理后的米糠膳食纤维(p<0.05),而脂质、蛋白质和淀粉的比例则较低。纤维素酶修饰 ERBDF 可显著降低(p<0.05)纤维素、半纤维素、淀粉和蛋白质含量,而相对木质素含量增加。纤维素酶修饰显著改善(p<0.05)持水力、持油力、溶胀能力、胆固醇吸收能力和葡萄糖吸附能力,同时降低乳化能力和葡萄糖透析迟滞指数。纤维样品经纤维素酶修饰后的物理化学和功能性质的变化归因于纤维表面孔隙率的增加、结晶度降低导致结合位点更大程度的暴露以及化学成分的变化。