Bu Tingting, Yu Yue, Kong Xiao, Wu Weicheng, Zhang Zhiguo, Hu Weiwei, Natallia Komarova, Cai Ming, Yang Kai, Sun Peilong
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
Moganshan Institute ZJUT, Deqing 313200, China.
Foods. 2025 Apr 9;14(8):1300. doi: 10.3390/foods14081300.
The effects of cellulase-xylanase synergistic treatment combined with twin-screw extrusion on the physicochemical, functional, and in vitro fermentation characteristics of buckwheat bran dietary fiber (BBDF) were investigated. Compared to single enzymatic hydrolysis, the synergetic modification was more effective in promoting the soluble DF (SDF) ratio (increased from 10.68% to 32.67%), functional properties, and prebiotic activities of BBDF and decreasing the insoluble DF (IDF) content. Under 0.6% (/) cellulase and xylanase with mild extrusion conditions (40-80 °C), the modified BBDF exhibited the highest capacities for glucose and cholesterol adsorption. FTIR and XRD experiments indicated that the enzymatic extrusion destroyed the intermolecular interactions of BBDF. Furthermore, enzymatically extruded BBDFs showed 2.2-fold higher short-chain fatty acid (SCFA) yields during in vitro fecal fermentation (total SCFAs: 87.8 mM vs. 40.0 mM in control), with butyrate production reaching 2.5 mM (+76.3%), among which the mildly extruded BBDFs exhibited superior prebiotic effects.
研究了纤维素酶-木聚糖酶协同处理结合双螺杆挤压对苦荞麸膳食纤维（BBDF）的物理化学、功能和体外发酵特性的影响。与单一酶水解相比，协同改性在提高BBDF的可溶性膳食纤维（SDF）比例（从10.68%提高到32.67%）、功能特性和益生元活性以及降低不溶性膳食纤维（IDF）含量方面更有效。在0.6%（/）纤维素酶和木聚糖酶以及温和挤压条件（40-80°C）下，改性后的BBDF表现出最高的葡萄糖和胆固醇吸附能力。傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）实验表明，酶促挤压破坏了BBDF的分子间相互作用。此外，酶促挤压的BBDF在体外粪便发酵过程中短链脂肪酸（SCFA）产量提高了2.2倍（总SCFAs：87.8 mM，对照为40.0 mM），丁酸盐产量达到2.5 mM（+76.3%），其中轻度挤压的BBDF表现出优异的益生元效果。
