Cheung Sai Man, Husain Ehab, Mallikourti Vasiliki, Masannat Yazan, Heys Steven, He Jiabao
Institute of Medical Sciences, School of Medicine, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK.
Pathology Department, Aberdeen Royal Infirmary, Aberdeen, UK.
Eur Radiol. 2021 Jun;31(6):3703-3711. doi: 10.1007/s00330-020-07502-4. Epub 2020 Dec 3.
Despite improved survival due to new treatments, the 10-year survival rate in patients with breast cancer is approximately 75%. Lymphovascular invasion (LVI), a prognostic marker independent from histological grade and stage, can only be fully determined at final histological examination. Lipid composition is deregulated in tumour via de novo lipogenesis, with alteration in lipogenic genes in LVI. We hypothesise alteration in lipid composition derived from novel non-invasive spectroscopy method is associated with LVI positivity.
Thirty female patients (age 39-78) with invasive ductal carcinoma were enrolled, with 13 LVI negative and 17 LVI positive. Saturated, monounsaturated, polyunsaturated fatty acids and triglycerides (SFA, MUFA, PUFA and TRG) were quantified from ex vivo breast tumours freshly excised from patients on a 3 T clinical MRI scanner, and proliferative activity marker Ki-67 and serotonin derived histologically.
There were significantly lower MUFA (p = 0.0189) in LVI positive (median: 0.37, interquartile range (IQR): 0.25-0.64) than negative (0.63, 0.49-0.96). There were significantly lower TRG (p = 0.0226) in LVI positive (1.32, 0.95-2.43) than negative (2.5, 1.92-4.15). There was no significant difference in SFA (p = 0.6009) or PUFA (p = 0.1641). There was no significant correlation between lipid composition against Ki-67 or serotonin, apart from a borderline negative correlation between PUFA and serotonin (r = - 0.3616, p = 0.0496).
Lipid composition might provide a biomarker to study lymphovascular invasion in breast cancer.
• Monounsaturated fatty acids in lymphovascular invasion (LVI) positive invasive breast carcinoma were significantly lower than that in LVI negative. • Triglycerides in LVI positive invasive breast carcinoma were significantly lower than that in LVI negative. • Lipid composition from MR spectroscopy reflects the rate of de novo lipogenesis and provides a potential biomarker independent from histological grade and stage.
尽管新治疗方法提高了生存率，但乳腺癌患者的10年生存率约为75%。淋巴管浸润（LVI）是一种独立于组织学分级和分期的预后标志物，只能在最终组织学检查时才能完全确定。肿瘤中的脂质成分通过从头脂肪生成而失调，LVI中脂肪生成基因发生改变。我们假设，源自新型非侵入性光谱法的脂质成分改变与LVI阳性相关。
招募了30名患有浸润性导管癌的女性患者（年龄39 - 78岁），其中13例LVI阴性，17例LVI阳性。在3T临床MRI扫描仪上，对从患者新鲜切除的离体乳腺肿瘤中饱和、单不饱和、多不饱和脂肪酸和甘油三酯（SFA、MUFA、PUFA和TRG）进行定量，并通过组织学方法检测增殖活性标志物Ki-67和血清素。
LVI阳性组（中位数：0.37，四分位间距（IQR）：0.25 - 0.64）的MUFA显著低于阴性组（0.63，0.49 - 0.96）（p = 0.0189）。LVI阳性组（1.32，0.95 - 2.43）的TRG显著低于阴性组（2.5，1.92 - 4.15）（p = 0.0226）。SFA（p = 0.6009）或PUFA（p = 0.1641）无显著差异。除了PUFA与血清素之间存在临界负相关（r = - 0.3616，p = 0.0496）外，脂质成分与Ki-67或血清素之间无显著相关性。
脂质成分可能为研究乳腺癌淋巴管浸润提供一种生物标志物。
• 淋巴管浸润（LVI）阳性的浸润性乳腺癌中的单不饱和脂肪酸显著低于LVI阴性者。• LVI阳性的浸润性乳腺癌中的甘油三酯显著低于LVI阴性者。• 磁共振波谱的脂质成分反映了从头脂肪生成的速率，并提供了一种独立于组织学分级和分期的潜在生物标志物。
